ORAI1 Monoclonal antibody
ORAI1 Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat, pig and More (2)
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
3C12F9
Cat no : 66223-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HL-60 cells, 4T1 cells, A375 cells, RAW 264.7 cells, pig spleen tissue, rat spleen tissue, pig thymus tissue, Raji cells, Ramos cells, Daudi cells, Jurkat cells, K-562 cells |
Positive IF/ICC detected in | Jurkat cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:30000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 21 publications below |
IF | See 5 publications below |
Product Information
66223-1-Ig targets ORAI1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human, mouse, rat, monkey, bovine |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | ORAI1 fusion protein Ag18050 相同性解析による交差性が予測される生物種 |
Full Name | ORAI calcium release-activated calcium modulator 1 |
Calculated molecular weight | 301 aa, 33 kDa |
Observed molecular weight | 32 kDa |
GenBank accession number | BC015369 |
Gene symbol | ORAI1 |
Gene ID (NCBI) | 84876 |
RRID | AB_2881614 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Store-operated calcium channels (SOCs) play essential functions in various physiological processes. Orai1 (also named CRACM1) is a critical molecular component of SOCs. Orai1 protein is a 32-55 kDa plasma membrane protein with 4 transmembrane domains. Human patients with a severe combinaed immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene.
Protocols
Product Specific Protocols | |
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WB protocol for ORAI1 antibody 66223-1-Ig | Download protocol |
IF protocol for ORAI1 antibody 66223-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Death Dis Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats | ||
Acta Pharmacol Sin Endothelial cell Orai1 is essential for endothelium-dependent contraction of mouse carotid arteries in normotensive and hypertensive mice | ||
Oncogenesis Blockage of Orai1-Nucleolin interaction meditated calcium influx attenuates breast cancer cells growth | ||
Int J Mol Sci Neuroprotective Effect of Antiapoptotic URG7 Protein on Human Neuroblastoma Cell Line SH-SY5Y | ||
Front Cell Dev Biol Transmembrane Protein Ttyh1 Maintains the Quiescence of Neural Stem Cells Through Ca2+/NFATc3 Signaling. | ||
J Innate Immun Mannan-Binding Lectin Reduces Epithelial-Mesenchymal Transition in Pulmonary Fibrosis via Inactivating the Store-Operated Calcium Entry Machinery. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Udesh (Verified Customer) (01-19-2023) | The Ab worked well for WB. 20ug protein loaded. Probed overnight at 4 deg.
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