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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ORAI1 Monoclonal antibody, PBS Only

ORAI1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C12F9

Cat no : 66223-1-PBS

Synonyms

ORAI1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ORAI1 Monoclonal antibody (66223-1-Ig)

    WB analysis using 66223-1-Ig (same clone as 66223-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66223-1-Ig (ORAI1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66223-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ORAI1 Monoclonal antibody (66223-1-Ig)

    WB analysis using 66223-1-Ig (same clone as 66223-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66223-1-Ig (ORAI1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66223-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A375 cells using ORAI1 Monoclonal antibody (66223-1-Ig)

    WB analysis of A375 using 66223-1-Ig (same clone as 66223-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 66223-1-Ig (ORAI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66223-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using ORAI1 Monoclonal antibody (66223-1-Ig)

    IF Staining of Jurkat using 66223-1-Ig (same clone as 66223-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ORAI1 antibody (66223-1-Ig, Clone: 3C12F9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66223-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66223-1-PBS targets ORAI1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ORAI1 fusion protein Ag18050 相同性解析による交差性が予測される生物種
Full Name ORAI calcium release-activated calcium modulator 1
Calculated molecular weight 301 aa, 33 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC015369
Gene symbol ORAI1
Gene ID (NCBI) 84876
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Store-operated calcium channels (SOCs) play essential functions in various physiological processes. Orai1 (also named CRACM1) is a critical molecular component of SOCs. Orai1 protein is a 32-55 kDa plasma membrane protein with 4 transmembrane domains. Human patients with a severe combinaed immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene.