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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated ORM2-Specific Monocl (CL488-66217)

    IF Staining of HepG2 using CL488-66217

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 ORM2-Specific antibody (CL488-66217, Clone: 4E9H10 ) at dilution of 1:200.

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CoraLite® Plus 488-conjugated ORM2-Specific Monoclonal antibody

ORM2-Specific Monoclonal Antibody for IF/ICC
Cat No. CL488-66217
Clone No.4E9H10

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Synonyms

AGP 2, AGP B, AGP B', AGP2, Alpha 1 acid glycoprotein 2, OMD 2, ORM2, orosomucoid 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66217 targets ORM2-Specific in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ORM2-Specific fusion protein Ag18984 相同性解析による交差性が予測される生物種
Full Name orosomucoid 2
Calculated molecular weight 24 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC015964
Gene Symbol ORM2
Gene ID (NCBI) 5005
RRIDAB_2919302
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP19652
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ORM2, also named as AGP2 and OMD 2, belongs to the calycin superfamily and Lipocalin family. It functions as transport protein in the blood stream. ORM2 binds various hydrophobic ligands in the interior of its beta-barrel domain. It also binds synthetic drugs and influences their distribution and availability. ORM2 appears to function in modulating the activity of the immune system during the acute-phase reaction. ORM2 is one of the conserved endoplasmic reticulum membrane proteins which regulating lipid homeostasis and protein quality control. This antibody can specially recognize ORM2. It recognizes a band about 45 kDa in human plasma which may be due to the glycosylation of ORM2 or the dimer formation of the protein.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ORM2-Specific antibody CL488-66217Download protocol
Standard Protocols
Click here to view our Standard Protocols