Validation Data Gallery
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Tested Applications
| Positive WB detected in | MCF-7 cells, HUVEC cells, U2OS cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL750-82932 targets OS9 in WB, FC (Intra) applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | OS9 fusion protein Ag0106 相同性解析による交差性が予測される生物種 |
| Full Name | osteosarcoma amplified 9, endoplasmic reticulum associated protein |
| Calculated molecular weight | 76 kDa |
| Observed molecular weight | 83-97 kDa |
| GenBank accession number | BC000532 |
| Gene Symbol | OS9 |
| Gene ID (NCBI) | 10956 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13438 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
OS-9 is a ubiquitously expressed ensoplasmic reticulum (ER)-associated protein originally identified as being amplified in certain osteosarcomas. It functions in ER quality control and ER-associated degradation (ERAD). There are three isoforms of this protein: OS-9.1, OS-9.2 and OS-9.3. The longest isoform, OS-9.1, contains 667 aa, OS-9.2 lacks aa 535-589, whereas OS-9.3 lacks aa 456-470 and 535-589. OS-9.1 and OS-9.2 are N-glycosylated, ubiquitously expressed in human tissues, and amplified in tumors.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 OS9 antibody CL750-82932 | Download protocol |
| FC protocol for CL Plus 750 OS9 antibody CL750-82932 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |