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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

OTUD6B Monoclonal antibody

OTUD6B Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D1G1

Cat no : 68450-1-Ig

Synonyms

DUBA5, DUBA 5, Deubiquitinase OTUD6B, CGI-77, CGI 77

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OTUD6B Monoclonal antibody (68450-1-Ig)

    WB analysis using 68450-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68450-1-Ig (OTUD6B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using OTUD6B Monoclonal antibody (68450-1-Ig)

    IF Staining of PC-3 using 68450-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using OTUD6B antibody (68450-1-Ig, Clone: 1D1G1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Tested Applications

Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68450-1-Ig targets OTUD6B in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen OTUD6B fusion protein Ag33607 相同性解析による交差性が予測される生物種
Full Name OTU domain containing 6B
Calculated molecular weight 293 aa, 34 kDa
Observed molecular weight 34-40 kDa
GenBank accession numberBC029760
Gene symbol OTUD6B
Gene ID (NCBI) 51633
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

OTUD6B, also known as DUBA5, belongs to a DUB subfamily characterized by an ovarian tumor domain (OTU). It's a functional deubiquitinating enzyme, a class of protease that specifically cleaves ubiquitin linkages. OTUD6B function may be connected to growth and proliferation. It has been reported that OTUD6B could significantly suppress HCC cells migration and metastasis. The calculated molecular weight of OTUD6B is 34 kDa, 68450-1-Ig can detect the band around 34 kDa.(PMID:32323143)

Protocols

Product Specific Protocols
WB protocol for OTUD6B antibody 68450-1-IgDownload protocol
IF protocol for OTUD6B antibody 68450-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols