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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OTUD6B Monoclonal antibody (68450-1-Ig)

    WB analysis using 68450-1-Ig (same clone as 68450-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68450-1-Ig (OTUD6B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68450-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using OTUD6B Monoclonal antibody (68450-1-Ig)

    IF Staining of PC-3 using 68450-1-Ig (same clone as 68450-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using OTUD6B antibody (68450-1-Ig, Clone: 1D1G1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68450-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50962-1

    Cytometric bead array standard curve of MP50962-1

    Cytometric bead array standard curve of MP50962-1, OTUD6B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68450-1-PBS. Detection antibody: 68450-2-PBS. Standard:Ag33607. Range: 0.098-50 ng/mL.

  • Cytometric bead array standard curve of MP50962-2

    Cytometric bead array standard curve of MP50962-2

    Cytometric bead array standard curve of MP50962-2, OTUD6B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68450-1-PBS. Detection antibody: 68450-3-PBS. Standard:Ag33607. Range: 0.098-25 ng/mL.

  • Cytometric bead array standard curve of MP50962-3

    Cytometric bead array standard curve of MP50962-3

    Cytometric bead array standard curve of MP50962-3, OTUD6B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68450-1-PBS. Detection antibody: 68450-4-PBS. Standard:Ag33607. Range: 0.098-12.5 ng/mL.

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OTUD6B Monoclonal antibody, PBS Only (Capture)

OTUD6B Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 68450-1-PBS
Clone No.1D1G1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

DUBA5, DUBA 5, Deubiquitinase OTUD6B, CGI-77, CGI 77

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68450-1-PBS targets OTUD6B as part of a matched antibody pair:

MP50962-1: 68450-1-PBS capture and 68450-2-PBS detection (validated in Cytometric bead array)

MP50962-2: 68450-1-PBS capture and 68450-3-PBS detection (validated in Cytometric bead array)

MP50962-3: 68450-1-PBS capture and 68450-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen OTUD6B fusion protein Ag33607 相同性解析による交差性が予測される生物種
Full Name OTU domain containing 6B
Calculated molecular weight 293 aa, 34 kDa
Observed molecular weight 34-40 kDa
GenBank accession numberBC029760
Gene Symbol OTUD6B
Gene ID (NCBI) 51633
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8N6M0
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

OTUD6B, also known as DUBA5, belongs to a DUB subfamily characterized by an ovarian tumor domain (OTU). It's a functional deubiquitinating enzyme, a class of protease that specifically cleaves ubiquitin linkages. OTUD6B function may be connected to growth and proliferation. It has been reported that OTUD6B could significantly suppress HCC cells migration and metastasis. The calculated molecular weight of OTUD6B is 34 kDa, 68450-1-Ig can detect the band around 34 kDa.(PMID:32323143)