- Featured Product
- KD/KO Validated
OXA1L Monoclonal antibody
OXA1L Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
4A2D9
Cat no : 66128-1-Ig
Synonyms
Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | T-47D cells, NIH/3T3 cells, HEK-293 cells, HeLa cells, HepG2 cells, L02 cells, Jurkat cells |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
Product Information
66128-1-Ig targets OXA1L in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | OXA1L fusion protein Ag13819 相同性解析による交差性が予測される生物種 |
| Full Name | oxidase (cytochrome c) assembly 1-like |
| Calculated molecular weight | 437 aa, 49 kDa |
| Observed molecular weight | 42 kDa |
| GenBank accession number | BC001669 |
| Gene symbol | OXA1L |
| Gene ID (NCBI) | 5018 |
| RRID | AB_2881527 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
OXA1L/Oxa1 is a member of the conserved Oxa1/YidC/Alb3 protein family and is required for cytochrome c oxidase assembly. The OXA1L protein was initially identified in yeast proteins involved in the biogenesis of the mitochondrial respiratory chain complexes. It locates in the inner membrane of mitochondria and may be involved in mediating the insertion of proteins from the matrix into the inner membrane.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for OXA1L antibody 66128-1-Ig | Download protocol |
| IHC protocol for OXA1L antibody 66128-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Rep EMRE Is a Matrix Ca(2+) Sensor that Governs Gatekeeping of the Mitochondrial Ca(2+) Uniporter. | ||
