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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OXA1L Monoclonal antibody (66128-1-Ig)

    WB analysis using 66128-1-Ig (same clone as 66128-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66128-1-Ig (OXA1L antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66128-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using OXA1L Monoclonal antibody (66128-1-Ig)

    WB analysis of HEK-293 using 66128-1-Ig (same clone as 66128-1-PBS)

    WB result of OXA1L antibody (66128-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OXA1L transfected HEK-293 cells. This data was developed using the same antibody clone with 66128-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using OXA1L Monoclonal antibody (66128-1-Ig)

    WB analysis of NIH/3T3 using 66128-1-Ig (same clone as 66128-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66128-1-Ig (OXA1L antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66128-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using OXA1L Monoclonal antibody (66128-1-Ig)

    IHC staining of human brain using 66128-1-Ig (same clone as 66128-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain slide using 66128-1-Ig (OXA1L Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66128-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using OXA1L Monoclonal antibody (66128-1-Ig)

    IHC staining of human brain using 66128-1-Ig (same clone as 66128-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain slide using 66128-1-Ig (OXA1L Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66128-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

OXA1L Monoclonal antibody, PBS Only

OXA1L Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66128-1-PBS
Clone No.4A2D9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Hsa, OXA1 like protein, OXA1Hs, OXA1L

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66128-1-PBS targets OXA1L in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen OXA1L fusion protein Ag13819 相同性解析による交差性が予測される生物種
Full Name oxidase (cytochrome c) assembly 1-like
Calculated molecular weight 437 aa, 49 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC001669
Gene Symbol OXA1L
Gene ID (NCBI) 5018
RRIDAB_2881527
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15070
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

OXA1L/Oxa1 is a member of the conserved Oxa1/YidC/Alb3 protein family and is required for cytochrome c oxidase assembly. The OXA1L protein was initially identified in yeast proteins involved in the biogenesis of the mitochondrial respiratory chain complexes. It locates in the inner membrane of mitochondria and may be involved in mediating the insertion of proteins from the matrix into the inner membrane.