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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OXCT1 Recombinant antibody (81011-1-RR)

    WB analysis using 81011-1-RR (same clone as 81011-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81011-1-RR (OXCT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81011-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using OXCT1 Recombinant antibody (81011-1-RR)

    WB analysis of HeLa using 81011-1-RR (same clone as 81011-1-PBS)

    WB result of OXCT1 antibody (81011-1-RR; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OXCT1 transfected HeLa cells. This data was developed using the same antibody clone with 81011-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using OXCT1 Recombinant antibody (81011-1-RR)

    IF Staining of HeLa using 81011-1-RR (same clone as 81011-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (81011-1-RR, Clone: 4K12 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81011-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SKOV-3 cells using OXCT1 Recombinant antibody (81011-1-RR)

    IF Staining of SKOV-3 using 81011-1-RR (same clone as 81011-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed SKOV-3 cells using OXCT1 antibody (81011-1-RR, Clone: 4K12 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81011-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using OXCT1 Recombinant antibody (81011-1-RR)

    IF Staining of HeLa using 81011-1-RR (same clone as 81011-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (81011-1-RR, Clone: 4K12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81011-1-PBS in a different storage buffer formulation.

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OXCT1 Recombinant antibody, PBS Only

OXCT1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 81011-1-PBS
Clone No.4K12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

3 oxoacid CoA transferase 1, 3-oxoacid CoA-transferase 1, 4K12, EC:2.8.3.5, OXCT

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81011-1-PBS targets OXCT1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen OXCT1 fusion protein Ag2818 相同性解析による交差性が予測される生物種
Full Name 3-oxoacid CoA transferase 1
Calculated molecular weight 520 aa, 56 kDa
Observed molecular weight56 kDa
GenBank accession numberBC009001
Gene Symbol OXCT1
Gene ID (NCBI) 5019
RRIDAB_2918924
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP55809
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

3-oxoacid-CoA transferase 1 (OXCT1), encoded by nuclear gene, is a mitochondrial CoA transferase required for ketone body degradation. It catalyzes the transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA. OXCT1 is expressed in brain, heart, and skeletal muscle, but not in liver. This antibody specifically recognizes endogenous OXCT1. (21209089)