• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

OXCT1 Recombinant antibody, PBS Only

OXCT1 Recombinant Antibody for WB, IF, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4K12

Cat no : 81011-1-PBS

Synonyms

3 oxoacid CoA transferase 1, OXCT, OXCT1, SCOT, SCOT s

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using OXCT1 Recombinant antibody (81011-1-RR)

    WB analysis using 81011-1-RR (same clone as 81011-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81011-1-RR (OXCT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81011-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using OXCT1 Recombinant antibody (81011-1-RR)

    WB analysis of HeLa using 81011-1-RR (same clone as 81011-1-PBS)

    WB result of OXCT1 antibody (81011-1-RR; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OXCT1 transfected HeLa cells. This data was developed using the same antibody clone with 81011-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using OXCT1 Recombinant antibody (81011-1-RR)

    IF Staining of HeLa using 81011-1-RR (same clone as 81011-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (81011-1-RR, Clone: 4K12 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81011-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SKOV-3 cells using OXCT1 Recombinant antibody (81011-1-RR)

    IF Staining of SKOV-3 using 81011-1-RR (same clone as 81011-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed SKOV-3 cells using OXCT1 antibody (81011-1-RR, Clone: 4K12 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81011-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81011-1-PBS targets OXCT1 in WB, IF, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen OXCT1 fusion protein Ag2818 相同性解析による交差性が予測される生物種
Full Name 3-oxoacid CoA transferase 1
Calculated molecular weight 520 aa, 56 kDa
Observed molecular weight56 kDa
GenBank accession numberBC009001
Gene symbol OXCT1
Gene ID (NCBI) 5019
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

3-oxoacid-CoA transferase 1 (OXCT1), encoded by nuclear gene, is a mitochondrial CoA transferase required for ketone body degradation. It catalyzes the transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA. OXCT1 is expressed in brain, heart, and skeletal muscle, but not in liver. This antibody specifically recognizes endogenous OXCT1. (21209089)