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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OXCT1 Recombinant antibody (81011-1-RR)

    WB analysis using 81011-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81011-1-RR (OXCT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using OXCT1 Recombinant antibody (81011-1-RR)

    WB analysis of HeLa using 81011-1-RR

    WB result of OXCT1 antibody (81011-1-RR; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OXCT1 transfected HeLa cells.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using OXCT1 Recombinant antibody (81011-1-RR)

    IF Staining of HeLa using 81011-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (81011-1-RR, Clone: 4K12 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using OXCT1 Recombinant antibody (81011-1-RR)

    IF Staining of HeLa using 81011-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (81011-1-RR, Clone: 4K12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of SKOV-3 cells using OXCT1 Recombinant antibody (81011-1-RR)

    IF Staining of SKOV-3 using 81011-1-RR

    Immunofluorescent analysis of (10% Formaldehyde) fixed SKOV-3 cells using OXCT1 antibody (81011-1-RR, Clone: 4K12 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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OXCT1 Recombinant antibody

OXCT1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 81011-1-RR
Clone No.4K12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

3 oxoacid CoA transferase 1, 3-oxoacid CoA-transferase 1, 4K12, EC:2.8.3.5, OXCT

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, mouse skeletal muscle, mouse brain, rat brain, mouse heart, rat heart
Positive IF/ICC detected inHeLa cells, SKOV-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81011-1-RR targets OXCT1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen OXCT1 fusion protein Ag2818 相同性解析による交差性が予測される生物種
Full Name 3-oxoacid CoA transferase 1
Calculated molecular weight 520 aa, 56 kDa
Observed molecular weight56 kDa
GenBank accession numberBC009001
Gene Symbol OXCT1
Gene ID (NCBI) 5019
RRIDAB_2918924
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP55809
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

3-oxoacid-CoA transferase 1 (OXCT1), encoded by nuclear gene, is a mitochondrial CoA transferase required for ketone body degradation. It catalyzes the transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA. OXCT1 is expressed in brain, heart, and skeletal muscle, but not in liver. This antibody specifically recognizes endogenous OXCT1. (21209089)

Protocols

Product Specific Protocols
WB protocol for OXCT1 antibody 81011-1-RRDownload protocol
IF protocol for OXCT1 antibody 81011-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols