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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated OXCT1 Recombinant antibody

OXCT1 Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4K12

Cat no : CL488-81011

Synonyms

SCOT, OXCT, EC:2.8.3.5, 4K12, 3-oxoacid CoA-transferase 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated OXCT1 Recombinant an (CL488-81011)

    IF Staining of HeLa using CL488-81011

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 OXCT1 antibody (CL488-81011, Clone: 4K12 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite® Plus 488-conjugated OXCT1 Recombinant an (CL488-81011)

    IF Staining of MCF-7 using CL488-81011

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 OXCT1 antibody (CL488-81011, Clone: 4K12 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite® Plus 488-conjugated OXCT1 Recombinant an (CL488-81011)

    FC experiment of MCF-7 using CL488-81011

    1X10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human OXCT1 (CL488-81011, Clone:4K12) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells, MCF-7 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-81011 targets OXCT1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen OXCT1 fusion protein Ag2818 相同性解析による交差性が予測される生物種
Full Name 3-oxoacid CoA transferase 1
Calculated molecular weight 520 aa, 56 kDa
Observed molecular weight56 kDa
GenBank accession numberBC009001
Gene symbol OXCT1
Gene ID (NCBI) 5019
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

3-oxoacid-CoA transferase 1 (OXCT1), encoded by nuclear gene, is a mitochondrial CoA transferase required for ketone body degradation. It catalyzes the transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA. OXCT1 is expressed in brain, heart, and skeletal muscle, but not in liver. This antibody specifically recognizes endogenous OXCT1. (21209089)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 OXCT1 antibody CL488-81011Download protocol
FC protocol for CL Plus 488 OXCT1 antibody CL488-81011Download protocol
Standard Protocols
Click here to view our Standard Protocols