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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Occludin Polyclonal antibody (13409-1-AP)

    WB analysis using 13409-1-AP (same clone as 13409-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13409-1-AP (Occludin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using Occludin Polyclonal antibody (13409-1-AP)

    WB analysis of mouse liver using 13409-1-AP (same clone as 13409-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 13409-1-AP (Occludin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using Occludin Polyclonal antibody (13409-1-AP)

    WB analysis of mouse liver using 13409-1-AP (same clone as 13409-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 13409-1-AP (Occludin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using Occludin Polyclonal antibody (13409-1-AP)

    WB analysis of mouse liver using 13409-1-AP (same clone as 13409-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 13409-1-AP (Occludin Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using Occludin Polyclonal antibody (13409-1-AP)

    WB analysis of human kidney using 13409-1-AP (same clone as 13409-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 13409-1-AP (OCLN antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using Occludin Polyclonal antibody (13409-1-AP)

    WB analysis of human liver using 13409-1-AP (same clone as 13409-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 13409-1-AP (OCLN antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using Occludin Polyclonal antibody (13409-1-AP)

    IP experiment of mouse liver using 13409-1-AP (same clone as 13409-1-PBS)

    IP result of anti-Occludin (IP:13409-1-AP, 3ug; Detection:13409-1-AP 1:500) with mouse liver tissue lysate 6400ug. This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Occludin Polyclonal antibody (13409-1-AP)

    IHC staining of human breast cancer using 13409-1-AP (same clone as 13409-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13409-1-AP (Occludin Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Occludin Polyclonal antibody (13409-1-AP)

    IHC staining of human breast cancer using 13409-1-AP (same clone as 13409-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13409-1-AP (Occludin Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Occludin Polyclonal antibody (13409-1-AP)

    IHC staining of human breast cancer using 13409-1-AP (same clone as 13409-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13409-1-AP (Occludin Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Occludin Polyclonal antibody (13409-1-AP)

    IHC staining of human breast cancer using 13409-1-AP (same clone as 13409-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13409-1-AP (Occludin Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Occludin Polyclonal antibody (13409-1-AP)

    IF Staining of MCF-7 using 13409-1-AP (same clone as 13409-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Occludin antibody (13409-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Occludin Polyclonal antibody (13409-1-AP)

    IF Staining of MCF-7 using 13409-1-AP (same clone as 13409-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Occludin antibody (13409-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13409-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Occludin Polyclonal antibody, PBS Only

Occludin Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 13409-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13409-1-PBS targets Occludin in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Occludin fusion protein Ag4057 相同性解析による交差性が予測される生物種
Full Name occludin
Calculated molecular weight 522 aa, 59 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC029886
Gene Symbol Occludin
Gene ID (NCBI) 4950
RRIDAB_2156308
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16625
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).