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Target

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated Osteocalcin/OCN Polyclonal antibody

Osteocalcin/OCN Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-23418

Synonyms

BGLAP, Osteocalcin, Gamma-carboxyglutamic acid-containing protein, Bone Gla protein, BGP

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using CoraLite®594-conjugated Osteocalcin Polyclonal ant (CL594-23418)

    IF Staining of NIH/3T3 using CL594-23418

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite®594 Osteocalcin antibody (CL594-23418) at dilution of 1:400.

  • Flow cytometry (FC) experiment of NIH/3T3 cells using CoraLite®594-conjugated Osteocalcin Polyclonal ant (CL594-23418)

    FC experiment of NIH/3T3 using CL594-23418

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.5 ug CoraLite®594 Anti-Human Osteocalcin (CL594-23418) (red), or 0.5 ug CoraLite®594-conjugated Rabbit IgG control Rabbit PolyAb (CL594-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-23418 targets Osteocalcin/OCN in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Osteocalcin/OCN fusion protein Ag20065 相同性解析による交差性が予測される生物種
Full Name bone gamma-carboxyglutamate (gla) protein
Calculated molecular weight 100 aa, 11 kDa
GenBank accession numberBC113432
Gene symbol Osteocalcin
Gene ID (NCBI) 632
RRIDAB_2919870
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL594 Osteocalcin/OCN antibody CL594-23418Download protocol
Standard Protocols
Click here to view our Standard Protocols