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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

P glycoprotein Monoclonal antibody

P glycoprotein Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1G6C2

Cat no : 67258-2-Ig

Synonyms

ABCB1, 1G6C2, EC:7.6.2.2, EC:7.6.2.1, ATP-dependent translocase ABCB1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using P glycoprotein Monoclonal antibody (67258-2-Ig)

    WB analysis of HeLa using 67258-2-Ig

    unboiled HeLa cells were subjected to SDS PAGE followed by western blot with 67258-2-Ig (ABCB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using P glycoprotein Monoclonal antibody (67258-2-Ig)

    WB analysis of SH-SY5Y using 67258-2-Ig

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67258-2-Ig (ABCB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using P glycoprotein Monoclonal antibody (67258-2-Ig)

    WB analysis of HepG2 using 67258-2-Ig

    unboiled HepG2 cells were subjected to SDS PAGE followed by western blot with 67258-2-Ig (ABCB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using P glycoprotein Monoclonal antibody (67258-2-Ig)

    IHC staining of human kidney using 67258-2-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67258-2-Ig (ABCB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of COLO 320 cells using P glycoprotein Monoclonal antibody (67258-2-Ig)

    IF Staining of COLO 320 using 67258-2-Ig

    Immunofluorescent analysis of (4% PFA) fixed COLO 320 cells using P glycoprotein antibody (67258-2-Ig, Clone: 1G6C2 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Tested Applications

Positive WB detected inunboiled HeLa cells, unboiled HepG2 cells, SH-SY5Y cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCOLO 320 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67258-2-Ig targets P glycoprotein in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen P glycoprotein fusion protein Ag17811 相同性解析による交差性が予測される生物種
Full Name ATP-binding cassette, sub-family B (MDR/TAP), member 1
Calculated molecular weight 1280 aa, 142 kDa
Observed molecular weight130-150 kDa
GenBank accession numberBC130424
Gene symbol P glycoprotein
Gene ID (NCBI) 5243
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ABCB1 (also known as P-gp/ P-glycoprotein, MDR1/multidrug-resistance 1) is a plasma membrane protein first discovered in multidrug resistant tumor cells. ABCB1 can extrude a large number of medically relevant compounds from cells and causes drug resistance. ABCB1 is expressed in a variety of human cancers as well as normal tissues including brain, and placenta. Overexpression of ABCB1 correlates with a negative prognosis in several types of cancer. For optimal WB detection, we recommend to avoid boiling the sample after lysis.

Protocols

Product Specific Protocols
WB protocol for P glycoprotein antibody 67258-2-IgDownload protocol
IHC protocol for P glycoprotein antibody 67258-2-IgDownload protocol
IF protocol for P glycoprotein antibody 67258-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols