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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

P glycoprotein Monoclonal antibody, PBS Only

P glycoprotein Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G6C2

Cat no : 67258-2-PBS

Synonyms

ABC20, ABCB1, CD243, CLCS, GP170, MDR1, Multidrug resistance protein 1, P glycoprotein 1, P GP, PGY1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using P glycoprotein Monoclonal antibody (67258-2-Ig)

    WB analysis of HeLa using 67258-2-Ig (same clone as 67258-2-PBS)

    unboiled HeLa cells were subjected to SDS PAGE followed by western blot with 67258-2-Ig (ABCB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67258-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using P glycoprotein Monoclonal antibody (67258-2-Ig)

    WB analysis of SH-SY5Y using 67258-2-Ig (same clone as 67258-2-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67258-2-Ig (ABCB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67258-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using P glycoprotein Monoclonal antibody (67258-2-Ig)

    WB analysis of HepG2 using 67258-2-Ig (same clone as 67258-2-PBS)

    unboiled HepG2 cells were subjected to SDS PAGE followed by western blot with 67258-2-Ig (ABCB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67258-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using P glycoprotein Monoclonal antibody (67258-2-Ig)

    IHC staining of human kidney using 67258-2-Ig (same clone as 67258-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67258-2-Ig (ABCB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67258-2-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67258-2-PBS targets P glycoprotein in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen P glycoprotein fusion protein Ag17811 相同性解析による交差性が予測される生物種
Full Name ATP-binding cassette, sub-family B (MDR/TAP), member 1
Calculated molecular weight 1280 aa, 142 kDa
Observed molecular weight130-150 kDa
GenBank accession numberBC130424
Gene symbol P glycoprotein
Gene ID (NCBI) 5243
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ABCB1 (also known as P-gp/ P-glycoprotein, MDR1/multidrug-resistance 1) is a plasma membrane protein first discovered in multidrug resistant tumor cells. ABCB1 can extrude a large number of medically relevant compounds from cells and causes drug resistance. ABCB1 is expressed in a variety of human cancers as well as normal tissues including brain, and placenta. Overexpression of ABCB1 correlates with a negative prognosis in several types of cancer. For optimal WB detection, we recommend to avoid boiling the sample after lysis.