P27; KIP1 Monoclonal antibody
P27; KIP1 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat and More (1)
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
3F12C10
Cat no : 67355-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
Product Information
67355-1-Ig targets P27; KIP1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | P27; KIP1 fusion protein Ag14634 相同性解析による交差性が予測される生物種 |
| Full Name | cyclin-dependent kinase inhibitor 1B (p27, Kip1) |
| Calculated molecular weight | 198 aa, 22 kDa |
| Observed molecular weight | 27 kDa |
| GenBank accession number | BC001971 |
| Gene symbol | p27 Kip1/CDKN1B |
| Gene ID (NCBI) | 1027 |
| RRID | AB_2882610 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
DKN1B, also named as P27 or KIP1, is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. P27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controlling cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Downregulation of P27 has been implicated in the progression of several malignancies, including lung cancer, hepatocellular carcinoma, salivary cancer, oral squamous cell carcinomas, and gastric cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for P27; KIP1 antibody 67355-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Hepatocell Carcinoma CYP2C8 Suppress Proliferation, Migration, Invasion and Sorafenib Resistance of Hepatocellular Carcinoma via PI3K/Akt/p27 kip1 Axis | ||
Cell Rep FBXO38 mediates FGL1 ubiquitination and degradation to enhance cancer immunity and suppress inflammation | ||
J Vasc Res Isolation and Cultivation of Vascular Smooth Muscle Cells from the Mouse Circle of Willis |
