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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

P27; KIP1 Monoclonal antibody, PBS Only

P27; KIP1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3F12C10

Cat no : 67355-1-PBS

Synonyms

CDKN1B, CDKN4, KIP1, MEN1B, MEN4, P27, P27; KIP1, P27KIP1

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using P27; KIP1 Monoclonal antibody (67355-1-Ig)

    WB analysis of Jurkat using 67355-1-Ig (same clone as 67355-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67355-1-Ig (P27; KIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67355-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using P27; KIP1 Monoclonal antibody (67355-1-Ig)

    WB analysis of HSC-T6 using 67355-1-Ig (same clone as 67355-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67355-1-Ig (P27; KIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67355-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using P27; KIP1 Monoclonal antibody (67355-1-Ig)

    WB analysis of NIH/3T3 using 67355-1-Ig (same clone as 67355-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67355-1-Ig (P27; KIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67355-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using P27; KIP1 Monoclonal antibody (67355-1-Ig)

    WB analysis of K-562 using 67355-1-Ig (same clone as 67355-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 67355-1-Ig (P27; KIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67355-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67355-1-PBS targets P27; KIP1 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen P27; KIP1 fusion protein Ag14634 相同性解析による交差性が予測される生物種
Full Name cyclin-dependent kinase inhibitor 1B (p27, Kip1)
Calculated molecular weight 198 aa, 22 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC001971
Gene symbol p27 Kip1/CDKN1B
Gene ID (NCBI) 1027
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

DKN1B, also named as P27 or KIP1, is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. P27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controlling cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Downregulation of P27 has been implicated in the progression of several malignancies, including lung cancer, hepatocellular carcinoma, salivary cancer, oral squamous cell carcinomas, and gastric cancer.