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  • Featured Product
  • KD/KO Validated

P62,SQSTM1 Monoclonal antibody, PBS Only

P62,SQSTM1 Monoclonal Antibody for WB, IP, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IP, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H5C1

Cat no : 66184-1-PBS

Synonyms

A170, EBIAP, ORCA, OSIL, p60, P62, P62/SQSTM1, P62;SQSTM1, p62B, PDB3, sequestosome 1, SQSTM1, Ubiquitin binding protein p62, ZIP3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using P62,SQSTM1 Monoclonal antibody (66184-1-Ig)

    WB analysis using 66184-1-Ig (same clone as 66184-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66184-1-Ig (P62,SQSTM1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66184-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U2OS cells using P62,SQSTM1 Monoclonal antibody (66184-1-Ig)

    WB analysis of U2OS using 66184-1-Ig (same clone as 66184-1-PBS)

    U2OS (WT and SQSTM1 KO) lysates prepared with RIPA buffer, 25 μg protein loaded. 66184-1-Ig incubated at 1:1000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 66184-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using P62,SQSTM1 Monoclonal antibody (66184-1-Ig)

    WB analysis of HEK-293 using 66184-1-Ig (same clone as 66184-1-PBS)

    WB result of P62,SQSTM1 antibody (66184-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P62/SQSTM1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66184-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using P62,SQSTM1 Monoclonal antibody (66184-1-Ig)

    WB analysis of K-562 using 66184-1-Ig (same clone as 66184-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 66184-1-Ig (P62/SQSTM1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66184-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of U2OS cells using P62,SQSTM1 Monoclonal antibody (66184-1-Ig)

    IP experiment of U2OS using 66184-1-Ig (same clone as 66184-1-PBS)

    U2OS lysates prepared and IP of SQSTM1 performed using 1.0 μg of 66184-1-Ig coupled to protein G- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 66184-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using P62,SQSTM1 Monoclonal antibody (66184-1-Ig)

    IHC staining of human lung cancer using 66184-1-Ig (same clone as 66184-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66184-1-Ig (P62,SQSTM1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66184-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using P62,SQSTM1 Monoclonal antibody (66184-1-Ig)

    IHC staining of human liver cancer using 66184-1-Ig (same clone as 66184-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66184-1-Ig (P62,SQSTM1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66184-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using P62,SQSTM1 Monoclonal antibody (66184-1-Ig)

    IHC staining of human endometrial cancer using 66184-1-Ig (same clone as 66184-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 66184-1-Ig (P62,SQSTM1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66184-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using P62,SQSTM1 Monoclonal antibody (66184-1-Ig)

    IHC staining of human colon cancer using 66184-1-Ig (same clone as 66184-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66184-1-Ig (P62,SQSTM1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66184-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using P62,SQSTM1 Monoclonal antibody (66184-1-Ig)

    IHC staining of human liver cancer using 66184-1-Ig (same clone as 66184-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66184-1-Ig (P62/SQSTM1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66184-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using P62,SQSTM1 Monoclonal antibody (66184-1-Ig)

    IF Staining of U2OS using 66184-1-Ig (same clone as 66184-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using P62/SQSTM1 antibody (66184-1-Ig, Clone: 1H5C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66184-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using P62,SQSTM1 Monoclonal antibody (66184-1-Ig)

    IF Staining of HepG2 using 66184-1-Ig (same clone as 66184-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed Starvation treated HepG2 cells using 66184-1-Ig(P62,SQSTM1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66184-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using P62,SQSTM1 Monoclonal antibody (66184-1-Ig)

    IF Staining of U2OS using 66184-1-Ig (same clone as 66184-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated U2OS cells using P62/SQSTM1 antibody (66184-1-Ig, Clone: 1H5C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66184-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using P62,SQSTM1 Monoclonal antibody (66184-1-Ig)

    FC experiment of Jurkat using 66184-1-Ig (same clone as 66184-1-PBS)

    1X10^6 Jurkat cells were stained with 0.20ug P62,SQSTM1 antibody (66184-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH. This data was developed using the same antibody clone with 66184-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66184-1-PBS targets P62,SQSTM1 in WB, IP, IF, FC, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen P62,SQSTM1 fusion protein Ag13131 相同性解析による交差性が予測される生物種
Full Name sequestosome 1
Calculated molecular weight 48 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC017222
Gene symbol SQSTM1
Gene ID (NCBI) 8878
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and sometimes aggregated proteins for autophagic degradation. As a autophagy-specific substrate, p62 is degraded during the autophagic process, which makes intracellular level of p62 as a marker for autophagy flux. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer. p62 is a component of inclusion bodies/ protein aggregates found in human diseases, including Huntington's disease, Alzheimer's disease, Parkinson's disease in the brain, and nephropathic cystinosis in kidney (PMID: 22074114, 22860231, 22714671). The molecular weight of p62 is predicted as 48/ 38 kDa, while western blot analyses using this antibody demonstrate the major band around 60-62 kDa in various tissues.