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P62,SQSTM1 抗体 49 文献

Mouse Monoclonal| カタログ番号: 66184-1-Ig |クローン番号: 1H5C1

注目製品 KD/KO 検証済

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Related Primary Antibodies

適用アプリケーション

アプリケーション:
WB, IP, IHC, IF, FC, CoIP, ELISA
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Images See all

P62,SQSTM1 Antibody WB HeLa cells 66184-1-Ig

Various lysates were subjected to SDS PAGE followed by western blot with 66184-1-Ig (P62,SQSTM1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

P62,SQSTM1 Antibody WB HEK-293 cells 66184-1-Ig

WB result of P62,SQSTM1 antibody (66184-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P62/SQSTM1 transfected HEK-293 cells.

P62,SQSTM1 Antibody IF U2OS cells 66184-1-Ig

Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using P62/SQSTM1 antibody (66184-1-Ig, Clone: 1H5C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

P62,SQSTM1 Antibody IHC human lung cancer tissue 66184-1-Ig

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66184-1-Ig (P62,SQSTM1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

P62,SQSTM1 Antibody FC Jurkat cells 66184-1-Ig

1X10^6 Jurkat cells were stained with 0.20ug P62,SQSTM1 antibody (66184-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

種特異性:
human, bovine, monkey
WB ポジティブ:
HeLa cells, HEK-293 cells, K-562 cells, MCF-7 cells, HepG2 cells, Jurkat cells, L02 cells, Raji cells
IHC ポジティブ:
human lung cancer tissue, human colon cancer tissue, human liver cancer tissue, human endometrial cancer tissue
IF ポジティブ:
U2OS cells, Starvation treated HepG2 cells, Chloroquine treated U2OS cells, mouse brain tissue, rat brain tissue
FC ポジティブ:
Jurkat cells
推奨希釈倍率:
WB : 1:5000-1:50000
IHC : 1:2000-1:8000
IF : 1:200-1:800

Protocols

Product-specific protocols

Standard Protocols

製品情報

由来:
Mouse
精製方法:
Protein A purification
アイソタイプ:
IgG2b
バッファー/保存方法:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

ターゲット情報

免疫原:
P62,SQSTM1 fusion protein Ag13131
正式名:
sequestosome 1
計算分子量:
48 kDa
検出分子量:
62 kDa
GenBank accession number:
BC017222
Gene ID (NCBI):
8878
遺伝子シンボル:
SQSTM1
別名:
A170, EBIAP, ORCA, OSIL, p60, P62, P62/SQSTM1, P62;SQSTM1, p62B, PDB3, sequestosome 1, SQSTM1, Ubiquitin binding protein p62, ZIP3

背景説明

Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and sometimes aggregated proteins for autophagic degradation. As a autophagy-specific substrate, p62 is degraded during the autophagic process, which makes intracellular level of p62 as a marker for autophagy flux. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer. p62 is a component of inclusion bodies/ protein aggregates found in human diseases, including Huntington's disease, Alzheimer's disease, Parkinson's disease in the brain, and nephropathic cystinosis in kidney (PMID: 22074114, 22860231, 22714671). The molecular weight of p62 is predicted as 48/ 38 kDa, while western blot analyses using this antibody demonstrate the major band around 60-62 kDa in various tissues.


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