- Featured Product
- KD/KO Validated
CoraLite®594-conjugated P62,SQSTM1 Monoclonal antibody
P62,SQSTM1 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1H5C1
Cat no : CL594-66184
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | HepG2 cells, Chloroquine treated U2OS cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL594-66184 targets P62,SQSTM1 in IF/ICC applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | P62,SQSTM1 fusion protein Ag13131 相同性解析による交差性が予測される生物種 |
Full Name | sequestosome 1 |
Calculated molecular weight | 48 kDa |
Observed molecular weight | 62 kDa |
GenBank accession number | BC017222 |
Gene symbol | P62/SQSTM1 |
Gene ID (NCBI) | 8878 |
RRID | AB_2883528 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and sometimes aggregated proteins for autophagic degradation. As a autophagy-specific substrate, p62 is degraded during the autophagic process, which makes intracellular level of p62 as a marker for autophagy flux. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer. p62 is a component of inclusion bodies/ protein aggregates found in human diseases, including Huntington's disease, Alzheimer's disease, Parkinson's disease in the brain, and nephropathic cystinosis in kidney (PMID: 22074114, 22860231, 22714671). The molecular weight of p62 is predicted as 48/ 38 kDa, while western blot analyses using this antibody demonstrate the major band around 60-62 kDa in various tissues.
Protocols
Product Specific Protocols | |
---|---|
IF protocol for CL594 P62,SQSTM1 antibody CL594-66184 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |