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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using P62/SQSTM1 Recombinant antibody (84826-1-RR)

    WB analysis using 84826-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84826-1-RR (P62/SQSTM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using P62/SQSTM1 Recombinant antibody (84826-1-RR)

    WB analysis of rat brain using 84826-1-RR

    rat brain tissue were subjected to SDS PAGE followed by western blot with 84826-1-RR (P62/SQSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using P62/SQSTM1 Recombinant antibody (84826-1-RR)

    WB analysis of NIH/3T3 using 84826-1-RR

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84826-1-RR (P62/SQSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using P62/SQSTM1 Recombinant antibody (84826-1-RR)

    WB analysis using 84826-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84826-1-RR (P62/SQSTM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of NIH/3T3 cells using P62/SQSTM1 Recombinant antibody (84826-1-RR)

    IP experiment of NIH/3T3 using 84826-1-RR

    IP result of anti-P62/SQSTM1 (IP:84826-1-RR, 4ug; Detection:84826-1-RR 1:4000) with NIH/3T3 cells lysate 1920 ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using P62/SQSTM1 Recombinant antibody (84826-1-RR)

    IHC staining of mouse brain using 84826-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84826-1-RR (P62/SQSTM1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using P62/SQSTM1 Recombinant antibody (84826-1-RR)

    IHC staining of mouse brain using 84826-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84826-1-RR (P62/SQSTM1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using P62/SQSTM1 Recombinant antibody (84826-1-RR)

    IF Staining of NIH/3T3 using 84826-1-RR

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using P62/SQSTM1 antibody (84826-1-RR, Clone: 241992C4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of NIH/3T3 cells using P62/SQSTM1 Recombinant antibody (84826-1-RR)

    FC experiment of NIH/3T3 using 84826-1-RR

    1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug P62/SQSTM1 Recombinant antibody (84826-1-RR, Clone:241992C4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84826-1-RR

    Affinity and Kinetic Characterization of 84826-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84826-1-RR against Mouse P62,SQSTM1 were performed. The affinity constant is 0.203 nM.

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P62/SQSTM1 Recombinant antibody

P62/SQSTM1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 84826-1-RR
Clone No.241992C4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

p62, Sqstm1, 241992C4, OSF 6, Osi

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNIH/3T3 cells, HeLa cells, mouse brain tissue, rat brain tissue, HepG2 cells, Jurkat cells, Raji cells, HEK-293T cells, MCF-7 cells, mouse liver tissue
Positive IP detected inNIH/3T3 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84826-1-RR targets P62/SQSTM1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen P62/SQSTM1 fusion protein Ag35064 相同性解析による交差性が予測される生物種
Full Name sequestosome 1
Calculated molecular weight48 kDa
Observed molecular weight 62 kDa
GenBank accession numberNM_011018
Gene Symbol P62/SQSTM1
Gene ID (NCBI) 18412
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ64337
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and aggregated proteins for autophagic degradation. p62 is degraded during the autophagic process, which makes its intracellular level a marker for autophagy progression. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer.

Protocols

Product Specific Protocols
WB protocol for P62/SQSTM1 antibody 84826-1-RRDownload protocol
IHC protocol for P62/SQSTM1 antibody 84826-1-RRDownload protocol
IF protocol for P62/SQSTM1 antibody 84826-1-RRDownload protocol
IP protocol for P62/SQSTM1 antibody 84826-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols