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Validation Data Gallery

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  • Western Blot (WB) analysis of rat brain tissue using P62/SQSTM1 Recombinant antibody (84826-1-RR)

    WB analysis of rat brain using 84826-1-RR (same clone as 84826-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 84826-1-RR (P62/SQSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84826-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using P62/SQSTM1 Recombinant antibody (84826-1-RR)

    WB analysis of NIH/3T3 using 84826-1-RR (same clone as 84826-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84826-1-RR (P62/SQSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84826-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using P62/SQSTM1 Recombinant antibody (84826-1-RR)

    WB analysis using 84826-1-RR (same clone as 84826-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84826-1-RR (P62/SQSTM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84826-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using P62/SQSTM1 Recombinant antibody (84826-1-RR)

    WB analysis using 84826-1-RR (same clone as 84826-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84826-1-RR (P62/SQSTM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84826-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01608-2

    Sandwich ELISA standard curve of MP01608-2

    Sandwich ELISA standard curve of MP01608-2, Mouse P62,Sqstm1 Recombinant Matched Antibody Pair - PBS only. 84826-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag35064. 84826-2-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL

  • Sample test of MP01608-2

    Sample test of MP01608-2

    The mean P62,Sqstm1 concentration was determined to be 257.13 ng/mL in mouse brain tissue extract based on a 1.6 mg/mL extract load and 598.93 ng/mL in NIH/3T3 cell extract based on a 1.5 mg/mL extract load.

  • Immunoprecipitation (IP) experiment of NIH/3T3 cells using P62/SQSTM1 Recombinant antibody (84826-1-RR)

    IP experiment of NIH/3T3 using 84826-1-RR (same clone as 84826-1-PBS)

    IP result of anti-P62/SQSTM1 (IP:84826-1-RR, 4ug; Detection:84826-1-RR 1:4000) with NIH/3T3 cells lysate 1920 ug. This data was developed using the same antibody clone with 84826-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using P62/SQSTM1 Recombinant antibody (84826-1-RR)

    IHC staining of mouse brain using 84826-1-RR (same clone as 84826-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84826-1-RR (P62/SQSTM1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84826-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using P62/SQSTM1 Recombinant antibody (84826-1-RR)

    IHC staining of mouse brain using 84826-1-RR (same clone as 84826-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84826-1-RR (P62/SQSTM1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84826-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using P62/SQSTM1 Recombinant antibody (84826-1-RR)

    IF Staining of NIH/3T3 using 84826-1-RR (same clone as 84826-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using P62/SQSTM1 antibody (84826-1-RR, Clone: 241992C4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84826-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of NIH/3T3 cells using P62/SQSTM1 Recombinant antibody (84826-1-RR)

    FC experiment of NIH/3T3 using 84826-1-RR (same clone as 84826-1-PBS)

    1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug P62/SQSTM1 Recombinant antibody (84826-1-RR, Clone:241992C4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84826-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01608-1

    Cytometric bead array standard curve of MP01608-1

    Cytometric bead array standard curve of MP01608-1, MOUSE P62,Sqstm1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84826-2-PBS. Detection antibody: 84826-1-PBS. Standard: Ag35064. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84826-1-RR

    Affinity and Kinetic Characterization of 84826-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84826-1-RR against Mouse P62,SQSTM1 were performed. The affinity constant is 0.203 nM.

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Mouse P62/SQSTM1 Recombinant antibody, PBS Only (Capture/Detector)

P62/SQSTM1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84826-1-PBS
Clone No.241992C4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

p62, Sqstm1, 241992C4, OSF 6, Osi

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84826-1-PBS targets P62/SQSTM1 as part of a matched antibody pair:

MP01608-1: 84826-2-PBS capture and 84826-1-PBS detection (validated in Cytometric bead array)

MP01608-2: 84826-1-PBS capture and 84826-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen P62/SQSTM1 fusion protein Ag35064 相同性解析による交差性が予測される生物種
Full Name sequestosome 1
Calculated molecular weight48 kDa
Observed molecular weight 62 kDa
GenBank accession numberNM_011018
Gene Symbol P62/SQSTM1
Gene ID (NCBI) 18412
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ64337
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and aggregated proteins for autophagic degradation. p62 is degraded during the autophagic process, which makes its intracellular level a marker for autophagy progression. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer.