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  • KD/KO Validated

CoraLite® Plus 488-conjugated PA2G4 Monoclonal antibody

PA2G4 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3G10D9

Cat no : CL488-66055

Synonyms

HG4 1, ErbB3-binding protein 1, ErbB3 binding protein 1, EBP1, Cell cycle protein p38-2G4 homolog

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated PA2G4 Monoclonal ant (CL488-66055)

    IF Staining of HeLa using CL488-66055

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 PA2G4 antibody (CL488-66055, Clone: 3G10D9 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66055 targets PA2G4 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PA2G4 fusion protein Ag7850 相同性解析による交差性が予測される生物種
Full Name proliferation-associated 2G4, 38kDa
Calculated molecular weight 44 kDa
Observed molecular weight 38-42 kDa, 48 kDa
GenBank accession numberBC001951
Gene symbol PA2G4
Gene ID (NCBI) 5036
RRIDAB_2883242
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PA2G4 (EBP1), first identified as an ErbB3 binding protein, is a 38 kDa protein that is widely expressed in cultured cells and tissues. The presence of several functional domains such as a nuclear localization sequence (NLS), LxxLL and LxCxE suggests the involvement of Ebp1 in cell signaling pathways and gene transcription regulation. This antibody is a mouse mono-clonal antibody raised against full length PA2G4 of human origin.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PA2G4 antibody CL488-66055Download protocol
Standard Protocols
Click here to view our Standard Protocols