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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PABPC1,PABP Monoclonal antibody

PABPC1,PABP Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3D8B5

Cat no : CL488-66809

Synonyms

PAB1, PABP, PABP 1, PABP1, PABPC1, PABPC2, PABPL1, Poly(A) binding protein 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CoraLite® Plus 488-conjugated PABPC1,PABP Monoclon (CL488-66809)

    IF Staining of HEK-293 using CL488-66809

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CL488-66809 (PABPC1,PABP antibody) at dilution of 1:100.

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Tested Applications

Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66809 targets PABPC1,PABP in IF/ICC applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PABPC1,PABP fusion protein Ag27860 相同性解析による交差性が予測される生物種
Full Name poly(A) binding protein, cytoplasmic 1
Calculated molecular weight 71 kDa
Observed molecular weight 71 kDa
GenBank accession numberBC015958
Gene symbol PABPC1
Gene ID (NCBI) 26986
RRIDAB_2883384
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation [PMID:21989405]. Polyadenylate-binding protein 1 (PABPC1) is a cytoplasmic-nuclear shuttling protein important for protein translation initiation, and both RNA processing and stability. In the cytoplasm, PABPC1 binds to the 3' poly(A) tail of eukaryotic mRNAs through its RNA-recognition motifs (RRM) and interacts with the N-terminus of eIF4G, part of the eIF4F complex associated with the 5' cap structure [PMID:20009508, 17381337].

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PABPC1,PABP antibody CL488-66809Download protocol
Standard Protocols
Click here to view our Standard Protocols