PAI-1 Monoclonal antibody
PAI-1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, pig and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1H4A5
Cat no : 66261-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human peripheral blood platelets, pig liver tissue, pig serum, HuH-7 cells, U-251 cells, SH-SY5Y cells |
Positive IHC detected in | human kidney tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 15 publications below |
IHC | See 2 publications below |
IF | See 3 publications below |
Product Information
66261-1-Ig targets PAI-1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, pig samples.
Tested Reactivity | human, mouse, pig |
Cited Reactivity | human, mouse, rat, pig |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | PAI-1 fusion protein Ag22411 相同性解析による交差性が予測される生物種 |
Full Name | serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 |
Calculated molecular weight | 402 aa, 45 kDa |
Observed molecular weight | 45-50 kDa |
GenBank accession number | BC010860 |
Gene symbol | PAI-1 |
Gene ID (NCBI) | 5054 |
RRID | AB_2881648 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SERPINE1, also named Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. PAI-1 is the principal inhibitor of tissue plasminogen activator (tPA) and uPA, and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. The mature 379-residue protein had a calculated unglycosylated molecular mass of 42.7 kD. Three putative glycosylation sites were found.
Protocols
Product Specific Protocols | |
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WB protocol for PAI-1 antibody 66261-1-Ig | Download protocol |
IHC protocol for PAI-1 antibody 66261-1-Ig | Download protocol |
IF protocol for PAI-1 antibody 66261-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Front Immunol Immunological characterization of stroke-heart syndrome and identification of inflammatory therapeutic targets | ||
Int J Mol Sci Identification of Impacted Pathways and Transcriptomic Markers as Potential Mediators of Pulmonary Fibrosis in Transgenic Mice Expressing Human IGFBP5. | ||
Am J Respir Cell Mol Biol ZMPSTE24 Regulates SARS-CoV-2 Spike Protein-enhanced Expression of Endothelial Plasminogen Activator Inhibitor-1. | ||
Clin Sci (Lond) Anti-anemia drug FG4592 retards the AKI-to-CKD transition by improving vascular regeneration and antioxidative capability. | ||
Phytomedicine Rhamnocitrin extracted from Nervilia fordii inhibited vascular endothelial activation via miR-185/STIM-1/SOCE/NFATc3. | ||
Cancer Biomark Weighted correlation network analysis identifies FN1, COL1A1 and SERPINE1 associated with the progression and prognosis of gastric cancer |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Mona (Verified Customer) (10-17-2021) | Paraffin-embedded hamster tissue from the lung. Antigen retrieval was performed with Tris-EDTA buffer (pH 9).
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