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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PAI-1 Monoclonal antibody, PBS Only

PAI-1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H4A5

Cat no : 66261-1-PBS

Synonyms

SERPINE1, SERPINE1, PAI-1, Plasminogen activator inhibitor 1, PAI1, PAI 1

Validation Data Gallery

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  • Western Blot (WB) analysis of human peripheral blood platelets using PAI-1 Monoclonal antibody (66261-1-Ig)

    WB analysis of human peripheral blood platelets using 66261-1-Ig (same clone as 66261-1-PBS)

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66261-1-Ig (PAI-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66261-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig liver tissue using PAI-1 Monoclonal antibody (66261-1-Ig)

    WB analysis of pig liver using 66261-1-Ig (same clone as 66261-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 66261-1-Ig (PAI-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66261-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig serum using PAI-1 Monoclonal antibody (66261-1-Ig)

    WB analysis of pig serum using 66261-1-Ig (same clone as 66261-1-PBS)

    pig serum were subjected to SDS PAGE followed by western blot with 66261-1-Ig (PAI-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66261-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using PAI-1 Monoclonal antibody (66261-1-Ig)

    WB analysis of SH-SY5Y using 66261-1-Ig (same clone as 66261-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66261-1-Ig (PAI-1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66261-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-251 cells using PAI-1 Monoclonal antibody (66261-1-Ig)

    WB analysis of U-251 using 66261-1-Ig (same clone as 66261-1-PBS)

    U-251 cells were subjected to SDS PAGE followed by western blot with 66261-1-Ig (PAI-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66261-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HuH-7 cells using PAI-1 Monoclonal antibody (66261-1-Ig)

    WB analysis of HuH-7 using 66261-1-Ig (same clone as 66261-1-PBS)

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 66261-1-Ig (PAI-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66261-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PAI-1 Monoclonal antibody (66261-1-Ig)

    IHC staining of human kidney using 66261-1-Ig (same clone as 66261-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66261-1-Ig (PAI-1 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66261-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PAI-1 Monoclonal antibody (66261-1-Ig)

    IHC staining of human kidney using 66261-1-Ig (same clone as 66261-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66261-1-Ig (PAI-1 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66261-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using PAI-1 Monoclonal antibody (66261-1-Ig)

    IHC staining of mouse lung using 66261-1-Ig (same clone as 66261-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66261-1-Ig (PAI-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66261-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using PAI-1 Monoclonal antibody (66261-1-Ig)

    IHC staining of mouse lung using 66261-1-Ig (same clone as 66261-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66261-1-Ig (PAI-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66261-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PAI-1 Monoclonal antibody (66261-1-Ig)

    IF Staining of HepG2 using 66261-1-Ig (same clone as 66261-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PAI-1 antibody (66261-1-Ig, Clone: 1H4A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66261-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66261-1-PBS targets PAI-1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PAI-1 fusion protein Ag22411 相同性解析による交差性が予測される生物種
Full Name serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
Calculated molecular weight 402 aa, 45 kDa
Observed molecular weight45-50 kDa
GenBank accession numberBC010860
Gene symbol PAI-1
Gene ID (NCBI) 5054
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SERPINE1, also named Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. PAI-1 is the principal inhibitor of tissue plasminogen activator (tPA) and uPA, and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. The mature 379-residue protein had a calculated unglycosylated molecular mass of 42.7 kD. Three putative glycosylation sites were found.