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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PAIP1 Monoclonal antibody

PAIP1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rabbit

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2H9D4

Cat no : 68042-1-Ig

Synonyms

PABP interacting protein 1, PAIP 1, PAIP1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PAIP1 Monoclonal antibody (68042-1-Ig)

    WB analysis using 68042-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 68042-1-Ig (PAIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PAIP1 Monoclonal antibody (68042-1-Ig)

    WB analysis using 68042-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68042-1-Ig (PAIP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of rabbit brain tissue using PAIP1 Monoclonal antibody (68042-1-Ig)

    WB analysis of rabbit brain using 68042-1-Ig

    rabbit brain tissue were subjected to SDS PAGE followed by western blot with 68042-1-Ig (PAIP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PAIP1 Monoclonal antibody (68042-1-Ig)

    IHC staining of human liver cancer using 68042-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68042-1-Ig (PAIP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue, A549 cells, rabbit brain tissue, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68042-1-Ig targets PAIP1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rabbit samples.

Tested Reactivity Human, mouse, rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PAIP1 fusion protein Ag16773 相同性解析による交差性が予測される生物種
Full Name poly(A) binding protein interacting protein 1
Calculated molecular weight 70 kDa
Observed molecular weight40-45kDa, 62kDa
GenBank accession numberBC005295
Gene symbol PAIP1
Gene ID (NCBI) 10605
RRIDAB_2918784
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PAIP1 is a PABP-binding protein that contains two distinct PABP-binding motifs (PAMs). PAM1 binds to RNA recognition motif 2 in the N terminus of PABP; PAM2, which is a conserved region comprising approximately 15 amino acids, binds to the PABC domain of PABP. PAIP1 acts as a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs.

Protocols

Product Specific Protocols
WB protocol for PAIP1 antibody 68042-1-IgDownload protocol
IHC protocol for PAIP1 antibody 68042-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols