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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PAIP1 Monoclonal antibody (68042-1-Ig)

    WB analysis using 68042-1-Ig (same clone as 68042-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 68042-1-Ig (PAIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68042-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PAIP1 Monoclonal antibody (68042-1-Ig)

    WB analysis using 68042-1-Ig (same clone as 68042-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68042-1-Ig (PAIP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68042-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rabbit brain tissue using PAIP1 Monoclonal antibody (68042-1-Ig)

    WB analysis of rabbit brain using 68042-1-Ig (same clone as 68042-1-PBS)

    rabbit brain tissue were subjected to SDS PAGE followed by western blot with 68042-1-Ig (PAIP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68042-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PAIP1 Monoclonal antibody (68042-1-Ig)

    IHC staining of human liver cancer using 68042-1-Ig (same clone as 68042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68042-1-Ig (PAIP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68042-1-PBS in a different storage buffer formulation.

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PAIP1 Monoclonal antibody, PBS Only

PAIP1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 68042-1-PBS
Clone No.2H9D4

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rabbit

Applications

WB, IHC, Indirect ELISA

Synonyms

PABP interacting protein 1, PAIP 1, PAIP1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68042-1-PBS targets PAIP1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rabbit samples.

Tested Reactivity Human, mouse, rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PAIP1 fusion protein Ag16773 相同性解析による交差性が予測される生物種
Full Name poly(A) binding protein interacting protein 1
Calculated molecular weight 70 kDa
Observed molecular weight40-45kDa, 62kDa
GenBank accession numberBC005295
Gene Symbol PAIP1
Gene ID (NCBI) 10605
RRIDAB_2918784
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H074
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PAIP1 is a PABP-binding protein that contains two distinct PABP-binding motifs (PAMs). PAM1 binds to RNA recognition motif 2 in the N terminus of PABP; PAM2, which is a conserved region comprising approximately 15 amino acids, binds to the PABC domain of PABP. PAIP1 acts as a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs.