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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated PAK6 Monoclonal antibody

PAK6 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3G8C1

Cat no : CL488-67562

Synonyms

p21 activated kinase 6, PAK 5, PAK 6, PAK5, PAK6

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CoraLite® Plus 488-conjugated PAK6 Monoclonal anti (CL488-67562)

    IF Staining of HEK-293 using CL488-67562

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 PAK6 antibody (CL488-67562, Clone: 3G8C1 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67562 targets PAK6 in IF/ICC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PAK6 fusion protein Ag27692 相同性解析による交差性が予測される生物種
Full Name p21 protein (Cdc42/Rac)-activated kinase 6
Calculated molecular weight 681 aa, 75 kDa
Observed molecular weight70-75 kDa
GenBank accession numberBC035596
Gene symbol PAK6
Gene ID (NCBI) 56924
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PAK6(p21-activated kinase 6) is a serine/threonine kinase belonging to the p21-activated kinase (PAK) family. .This protein plays a role in the regulation of gene transcription and may protect cells from apoptosis through phosphorylation of BAD. Northern blot analysis revealed that PAK6 is highly expressed in testis and prostate tissues (PMID:11278661).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PAK6 antibody CL488-67562Download protocol
Standard Protocols
Click here to view our Standard Protocols