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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PAK7 Recombinant antibody (82935-1-RR)

    WB analysis using 82935-1-RR (same clone as 82935-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82935-1-RR (PAK7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82935-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PAK7 Recombinant antibody (82935-1-RR)

    WB analysis of HeLa using 82935-1-RR (same clone as 82935-1-PBS)

    WB result of PAK7 antibody (82935-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PAK7 transfected HeLa cells. This data was developed using the same antibody clone with 82935-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using PAK7 Recombinant antibody (82935-1-RR)

    IF Staining of U-251 using 82935-1-RR (same clone as 82935-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using PAK7 antibody (82935-1-RR, Clone: 230201A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 82935-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of K-562 cells using PAK7 Recombinant antibody (82935-1-RR)

    FC experiment of K-562 using 82935-1-RR (same clone as 82935-1-PBS)

    (blue)1x10^6 K-562 cells were intracellularly stained with 0.25 ug Anti-Human PAK7 (82935-1-RR, Clone:230201A10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82935-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using PAK7 Recombinant antibody (82935-1-RR)

    FC experiment of HeLa using 82935-1-RR (same clone as 82935-1-PBS)

    (blue)1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human PAK7 (82935-1-RR, Clone:230201A10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82935-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

PAK7 Recombinant antibody, PBS Only

PAK7 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82935-1-PBS
Clone No.230201A10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

PAK4/6/7, PAK 5, p21-activated kinase 7, p21-activated kinase 5, KIAA1264

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82935-1-PBS targets PAK7 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PAK7 fusion protein Ag29190 相同性解析による交差性が予測される生物種
Full Name p21 protein (Cdc42/Rac)-activated kinase 7
Calculated molecular weight 719 aa, 81 kDa
Observed molecular weight70 kDa
GenBank accession numberBC024179
Gene Symbol PAK7
Gene ID (NCBI) 57144
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9P286
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PAK7, also known as PAK5, is a newly found but scarcely researched type II PAKs (PAK4, 5, 6). PAK7 was originally found in the brain, which promotes filopodia formation in nerve cells. Recently, studies have found that the expression level of PAK7 significantly increased in colorectal cancer, ovarian cancer, gastric cancer, neuroglioma, hepatocellular carcinoma, pancreatic cancer, osteosarcoma, and breast cancer. PAK7 plays an important role in tumorigenesis, invasion, and metastasis, mainly related to its mechanism of regulating cytoskeleton, inhibiting apoptosis, and promoting proliferation. The calculated molecular weight of PAK7 is 80 kDa (PMID: 29805709, PMID: 34165002).