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PAK7 Recombinant antibody
PAK7 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF/ICC, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
230201A10
Cat no : 82935-1-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, SH-SY5Y cells |
Positive IF/ICC detected in | U-251 cells |
Positive FC (Intra) detected in | K-562 cells, HeLa cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
82935-1-RR targets PAK7 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | PAK7 fusion protein Ag29190 相同性解析による交差性が予測される生物種 |
Full Name | p21 protein (Cdc42/Rac)-activated kinase 7 |
Calculated molecular weight | 719 aa, 81 kDa |
Observed molecular weight | 70 kDa |
GenBank accession number | BC024179 |
Gene symbol | PAK7 |
Gene ID (NCBI) | 57144 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PAK7, also known as PAK5, is a newly found but scarcely researched type II PAKs (PAK4, 5, 6). PAK7 was originally found in the brain, which promotes filopodia formation in nerve cells. Recently, studies have found that the expression level of PAK7 significantly increased in colorectal cancer, ovarian cancer, gastric cancer, neuroglioma, hepatocellular carcinoma, pancreatic cancer, osteosarcoma, and breast cancer. PAK7 plays an important role in tumorigenesis, invasion, and metastasis, mainly related to its mechanism of regulating cytoskeleton, inhibiting apoptosis, and promoting proliferation. The calculated molecular weight of PAK7 is 80 kDa (PMID: 29805709, PMID: 34165002).
Protocols
Product Specific Protocols | |
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WB protocol for PAK7 antibody 82935-1-RR | Download protocol |
IF protocol for PAK7 antibody 82935-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |