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PAPPA Monoclonal antibody

PAPPA Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G5A7

Cat no : 66962-1-Ig

Synonyms

ASBABP2, DIPLA1, IGF dependent IGFBP 4 protease, IGFBP 4ase, PAPA, PAPP A, PAPPA, PAPP-A, PAPPA1, Pappalysin 1, Pappalysin-1

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using PAPPA Monoclonal antibody (66962-1-Ig)

    WB analysis of human placenta using 66962-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66962-1-Ig (PAPPA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using PAPPA Monoclonal antibody (66962-1-Ig)

    WB analysis of MCF-7 using 66962-1-Ig

    WB result of PAPPA antibody (66962-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PAPPA transfected MCF-7 cells.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PAPPA Monoclonal antibody (66962-1-Ig)

    IHC staining of human placenta using 66962-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66962-1-Ig (PAPPA antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using PAPPA Monoclonal antibody (66962-1-Ig)

    IHC staining of human placenta using 66962-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66962-1-Ig (PAPPA antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inhuman placenta tissue, MCF-7 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:4000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66962-1-Ig targets PAPPA in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PAPPA fusion protein Ag11863 相同性解析による交差性が予測される生物種
Full Name pregnancy-associated plasma protein A, pappalysin 1
Calculated molecular weight 1627 aa, 181 kDa
Observed molecular weight 180-220 kDa
GenBank accession numberBC078657
Gene symbol PAPPA
Gene ID (NCBI) 5069
RRIDAB_2882285
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Pregnancy-associated plasma protein A (PAPPA) is a metalloproteinase secreted by human placenta, and it can interact with IGF-binding proteins (IGFBPs) to regulate the proteolysis and the bioavailability of insulin-like growth factor (IGF). Circulating PAPPA represents a consolidated biomarker in combined first-trimester screening tests, allowing an early prenatal diagnosis of chromosomal abnormalities, preeclampsia, and intrauterine growth restriction risk. PAPPA is highly expressed in placenta and pregnancy serum (PMID: 34974815, 34974815).

Protocols

Product Specific Protocols
WB protocol for PAPPA antibody 66962-1-IgDownload protocol
IHC protocol for PAPPA antibody 66962-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Oncol

Cancer-associated fibroblasts educate normal fibroblasts to facilitate cancer cell spreading and T cell suppression.

Authors - Go Itoh
View Article