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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using PAPPA Monoclonal antibody (66962-1-Ig)

    WB analysis of MCF-7 using 66962-1-Ig (same clone as 66962-1-PBS)

    WB result of PAPPA antibody (66962-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PAPPA transfected MCF-7 cells. This data was developed using the same antibody clone with 66962-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using PAPPA Monoclonal antibody (66962-1-Ig)

    WB analysis of human placenta using 66962-1-Ig (same clone as 66962-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66962-1-Ig (PAPPA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66962-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PAPPA Monoclonal antibody (66962-1-Ig)

    IHC staining of human placenta using 66962-1-Ig (same clone as 66962-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66962-1-Ig (PAPPA antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66962-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PAPPA Monoclonal antibody (66962-1-Ig)

    IHC staining of human placenta using 66962-1-Ig (same clone as 66962-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66962-1-Ig (PAPPA antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66962-1-PBS in a different storage buffer formulation.

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  • KD/KO Validated

PAPPA Monoclonal antibody, PBS Only

PAPPA Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66962-1-PBS
Clone No.1G5A7

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Synonyms

ASBABP2, DIPLA1, IGF dependent IGFBP 4 protease, IGFBP 4ase, PAPA, PAPP A, PAPPA, PAPP-A, PAPPA1, Pappalysin 1, Pappalysin-1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66962-1-PBS targets PAPPA in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PAPPA fusion protein Ag11863 相同性解析による交差性が予測される生物種
Full Name pregnancy-associated plasma protein A, pappalysin 1
Calculated molecular weight 1627 aa, 181 kDa
Observed molecular weight 180-220 kDa
GenBank accession numberBC078657
Gene Symbol PAPPA
Gene ID (NCBI) 5069
RRIDAB_2882285
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13219
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Pregnancy-associated plasma protein A (PAPPA) is a metalloproteinase secreted by human placenta, and it can interact with IGF-binding proteins (IGFBPs) to regulate the proteolysis and the bioavailability of insulin-like growth factor (IGF). Circulating PAPPA represents a consolidated biomarker in combined first-trimester screening tests, allowing an early prenatal diagnosis of chromosomal abnormalities, preeclampsia, and intrauterine growth restriction risk. PAPPA is highly expressed in placenta and pregnancy serum (PMID: 34974815, 34974815).