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CoraLite® Plus 488-conjugated PARD3 Polyclonal antibody
PARD3 Polyclonal Antibody for IF-P
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-11085
Synonyms
Validation Data Gallery
Tested Applications
Positive IF-P detected in | mouse colon tissue |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-11085 targets PARD3 in IF-P applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PARD3 fusion protein Ag1565 相同性解析による交差性が予測される生物種 |
Full Name | par-3 partitioning defective 3 homolog (C. elegans) |
Calculated molecular weight | 151 kDa |
Observed molecular weight | 180 kDa, 140-150 kDa, 100 kDa |
GenBank accession number | BC011711 |
Gene symbol | PARD3 |
Gene ID (NCBI) | 56288 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PARD3 (also known as ASIP, Par3, or Bazooka) is one of PARD proteins which are essential for asymmetric cell division and polarized growth. PARD3 is involved in the establishment of cell polarity and in the asymmetric cytokinesis. It plays a role in tight junctions at epithelial cell-cell contacts. PARD3 has three splice isoforms at 100 kDa, 150 kDa, and 180 kDa. This polyclonal antibody raised against C-terminal 281 amino acids of human PARD3 recognizes these three isoforms.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 PARD3 antibody CL488-11085 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |