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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PAX6 Recombinant antibody (84345-5-RR)

    WB analysis using 84345-5-RR (same clone as 84345-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84345-5-RR (PAX6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PAX6 Recombinant antibody (84345-5-RR)

    IHC staining of mouse brain using 84345-5-RR (same clone as 84345-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84345-5-RR (PAX6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PAX6 Recombinant antibody (84345-5-RR)

    IHC staining of mouse brain using 84345-5-RR (same clone as 84345-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84345-5-RR (PAX6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using PAX6 Recombinant antibody (84345-5-RR)

    IHC staining of mouse pancreas using 84345-5-RR (same clone as 84345-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84345-5-RR (PAX6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using PAX6 Recombinant antibody (84345-5-RR)

    IHC staining of mouse pancreas using 84345-5-RR (same clone as 84345-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84345-5-RR (PAX6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using PAX6 Recombinant antibody (84345-5-RR)

    IHC staining of mouse eye using 84345-5-RR (same clone as 84345-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 84345-5-RR (PAX6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using PAX6 Recombinant antibody (84345-5-RR)

    IHC staining of mouse eye using 84345-5-RR (same clone as 84345-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 84345-5-RR (PAX6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using PAX6 Recombinant antibody (84345-5-RR)

    IF Staining of mouse eye using 84345-5-RR (same clone as 84345-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PAX6 antibody (84345-5-RR, Clone: 241521G1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using PAX6 Recombinant antibody (84345-5-RR)

    IF Staining of mouse eye using 84345-5-RR (same clone as 84345-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PAX6 antibody (84345-5-RR, Clone: 241521G1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using PAX6 Recombinant antibody (84345-5-RR)

    IF Staining of mouse brain using 84345-5-RR (same clone as 84345-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using PAX6 antibody (84345-5-RR, Clone: 241521G1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U-251 cells using PAX6 Recombinant antibody (84345-5-RR)

    FC experiment of U-251 using 84345-5-RR (same clone as 84345-5-PBS)

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug PAX6 Recombinant antibody (84345-5-RR, Clone:241521G1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84345-5-RR

    Affinity and Kinetic Characterization of 84345-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84345-5-RR against Human PAX6 were performed. The affinity constant is 40.0 pM.

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PAX6 Recombinant antibody, PBS Only

PAX6 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA
Cat No. 84345-5-PBS
Clone No.241521G1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Synonyms

Pax-6, PAX 6, Paired box protein Pax-6, Paired box protein Pax 6, paired box 6

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84345-5-PBS targets PAX6 in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PAX6 fusion protein Ag2984 相同性解析による交差性が予測される生物種
Full Name paired box 6
Calculated molecular weight 47 kDa
Observed molecular weight47 kDa
GenBank accession numberBC011953
Gene Symbol PAX6
Gene ID (NCBI) 5080
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP26367
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PAX6, a paired domain and homeodomain-containing transcription factor. Interaction with TRIM11 leads to ubiquitination of PAX6 and its proteasomal degradation. PAX6 is one of the earliest genes expressed in the eye field and considered a master control gene for retinal and eye development. PAX6 also regulates the development of the olfactory, central nervous systems, pituitary, and pancreas. PAX6 mutations can cause complex ocular disorders such as aniridia and Peter's anomaly.