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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PAX6 Recombinant antibody

PAX6 Recombinant Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

241521G1

Cat no : 84345-5-RR

Synonyms

Pax-6, PAX 6, Paired box protein Pax-6, Paired box protein Pax 6, paired box 6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PAX6 Recombinant antibody (84345-5-RR)

    WB analysis using 84345-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84345-5-RR (PAX6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PAX6 Recombinant antibody (84345-5-RR)

    IHC staining of mouse brain using 84345-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84345-5-RR (PAX6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PAX6 Recombinant antibody (84345-5-RR)

    IHC staining of mouse brain using 84345-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84345-5-RR (PAX6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using PAX6 Recombinant antibody (84345-5-RR)

    IHC staining of mouse pancreas using 84345-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84345-5-RR (PAX6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using PAX6 Recombinant antibody (84345-5-RR)

    IHC staining of mouse pancreas using 84345-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84345-5-RR (PAX6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse eye tissue using PAX6 Recombinant antibody (84345-5-RR)

    IHC staining of mouse eye using 84345-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 84345-5-RR (PAX6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse eye tissue using PAX6 Recombinant antibody (84345-5-RR)

    IHC staining of mouse eye using 84345-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 84345-5-RR (PAX6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using PAX6 Recombinant antibody (84345-5-RR)

    IF Staining of mouse eye using 84345-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PAX6 antibody (84345-5-RR, Clone: 241521G1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using PAX6 Recombinant antibody (84345-5-RR)

    IF Staining of mouse eye using 84345-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PAX6 antibody (84345-5-RR, Clone: 241521G1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using PAX6 Recombinant antibody (84345-5-RR)

    IF Staining of mouse brain using 84345-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using PAX6 antibody (84345-5-RR, Clone: 241521G1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84345-5-RR

    Affinity and Kinetic Characterization of 84345-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84345-5-RR against Human PAX6 were performed. The affinity constant is 40.0 pM.

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Tested Applications

Positive WB detected inHeLa cells, K-562 cells, Neuro-2a cells
Positive IHC detected inmouse brain tissue, mouse eye tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse eye tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84345-5-RR is PAX6 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PAX6 fusion protein Ag2984 相同性解析による交差性が予測される生物種
Full Name paired box 6
Calculated molecular weight 47 kDa
Observed molecular weight47 kDa
GenBank accession numberBC011953
Gene symbol PAX6
Gene ID (NCBI) 5080
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PAX6, a paired domain and homeodomain-containing transcription factor. Interaction with TRIM11 leads to ubiquitination of PAX6 and its proteasomal degradation. PAX6 is one of the earliest genes expressed in the eye field and considered a master control gene for retinal and eye development. PAX6 also regulates the development of the olfactory, central nervous systems, pituitary, and pancreas. PAX6 mutations can cause complex ocular disorders such as aniridia and Peter's anomaly.

Protocols

Product Specific Protocols
WB protocol for PAX6 antibody 84345-5-RRDownload protocol
IHC protocol for PAX6 antibody 84345-5-RRDownload protocol
IF protocol for PAX6 antibody 84345-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols