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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PAX8 Recombinant antibody (80756-1-RR)

    WB analysis using 80756-1-RR (same clone as 80756-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80756-1-RR (PAX8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80756-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PAX8 Recombinant antibody (80756-1-RR)

    IHC staining of human ovary tumor using 80756-1-RR (same clone as 80756-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80756-1-RR (PAX8 antibody) at dilution of 1:8300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80756-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PAX8 Recombinant antibody (80756-1-RR)

    IHC staining of human ovary tumor using 80756-1-RR (same clone as 80756-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80756-1-RR (PAX8 antibody) at dilution of 1:8300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80756-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using PAX8 Recombinant antibody (80756-1-RR)

    IHC staining of human renal cell carcinoma using 80756-1-RR (same clone as 80756-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 80756-1-RR (PAX8 antibody) at dilution of 1:8300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80756-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using PAX8 Recombinant antibody (80756-1-RR)

    IHC staining of human renal cell carcinoma using 80756-1-RR (same clone as 80756-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 80756-1-RR (PAX8 antibody) at dilution of 1:8300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80756-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using PAX8 Recombinant antibody (80756-1-RR)

    IHC staining of human ovary cancer using 80756-1-RR (same clone as 80756-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 80756-1-RR (PAX8 antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80756-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using PAX8 Recombinant antibody (80756-1-RR)

    IHC staining of mouse kidney using 80756-1-RR (same clone as 80756-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 80756-1-RR (PAX8 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80756-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using PAX8 Recombinant antibody (80756-1-RR)

    IHC staining of mouse kidney using 80756-1-RR (same clone as 80756-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 80756-1-RR (PAX8 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80756-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using PAX8 Recombinant antibody (80756-1-RR)

    IHC staining of human thyroid cancer using 80756-1-RR (same clone as 80756-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 80756-1-RR (PAX8 antibody) at dilution of 1:8300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80756-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using PAX8 Recombinant antibody (80756-1-RR)

    IHC staining of human thyroid cancer using 80756-1-RR (same clone as 80756-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 80756-1-RR (PAX8 antibody) at dilution of 1:8300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80756-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SKOV-3 cells using PAX8 Recombinant antibody (80756-1-RR)

    IF Staining of SKOV-3 using 80756-1-RR (same clone as 80756-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using PAX8 antibody (80756-1-RR, Clone: 6B14 ) at dilution of 1:1300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80756-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80756-1-RR

    Affinity and Kinetic Characterization of 80756-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80756-1-RR against Human PAX8 were performed. The affinity constant is below 1 pM.

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PAX8 Recombinant antibody, PBS Only

PAX8 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 80756-1-PBS
Clone No.6B14

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

PAX-8, PAX 8, Paired box protein Pax-8, Paired box protein Pax 8, paired box 8

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80756-1-PBS targets PAX8 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PAX8 fusion protein Ag0306 相同性解析による交差性が予測される生物種
Full Name paired box 8
Calculated molecular weight 48 kDa
Observed molecular weight48 kDa, 58 kDa
GenBank accession numberBC001060
Gene Symbol PAX8
Gene ID (NCBI) 7849
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ06710
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinoma.