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PAX8 Recombinant antibody

PAX8 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 80756-1-RR
Clone No.6B14

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

PAX-8, PAX 8, Paired box protein Pax-8, Paired box protein Pax 8, paired box 8

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inSKOV-3 cells
Positive IHC detected inhuman ovary tumor tissue, human ovary cancer tissue, human renal cell carcinoma tissue, human thyroid cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSKOV-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)/ICCIF/ICC : 1:650-1:2600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80756-1-RR targets PAX8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PAX8 fusion protein Ag0306 相同性解析による交差性が予測される生物種
Full Name paired box 8
Calculated molecular weight 48 kDa
Observed molecular weight48 kDa, 58 kDa
GenBank accession numberBC001060
Gene Symbol PAX8
Gene ID (NCBI) 7849
RRIDAB_2923693
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ06710
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinoma.

Protocols

Product Specific Protocols
WB protocol for PAX8 antibody 80756-1-RRDownload protocol
IHC protocol for PAX8 antibody 80756-1-RRDownload protocol
IF protocol for PAX8 antibody 80756-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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