Biotin-conjugated PAX8 Monoclonal antibody
PAX8 Monoclonal Antibody for IHC
Host / Isotype
Mouse / IgG2b
Reactivity
human
Applications
IHC
Conjugate
Biotin
CloneNo.
4H7B3
Cat no : Biotin-60145
Synonyms
Validation Data Gallery
Tested Applications
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
Biotin-60145 targets PAX8 in IHC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | PAX8 fusion protein Ag0306 相同性解析による交差性が予測される生物種 |
| Full Name | paired box 8 |
| Calculated molecular weight | 48 kDa |
| GenBank accession number | BC001060 |
| Gene symbol | PAX8 |
| Gene ID (NCBI) | 7849 |
| RRID | AB_2883064 |
| Conjugate | Biotin |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas.
PAX8 has 5 isoforms with calculated MW 31kd, 34kd, 41kd, 43kd and 48kd. And there is a 60kd band corresponding to the electrophoretic mobility of PAX8 (PMID:15650356).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Biotin PAX8 antibody Biotin-60145 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
