CoraLite® Plus 488-conjugated PBLD Monoclonal antibody
PBLD Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
human, pig
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1G11D4
Cat no : CL488-68317
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-68317 targets PBLD in IF/ICC applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | PBLD fusion protein Ag23827 相同性解析による交差性が予測される生物種 |
| Full Name | phenazine biosynthesis-like protein domain containing |
| Observed molecular weight | 30 kDa |
| GenBank accession number | BC009738 |
| Gene symbol | PBLD |
| Gene ID (NCBI) | 64081 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PBLD, also termed as MAWBP, was first identified from a human liver cDNA library using a yeast two-hybrid technique by Iriyama et al. As the only representative of phenazine biosynthesis-like protein family, PBLD is widely expressed in human tissues, such as brain, heart, lung, liver, pancreas, kidney, and placenta. Its expression is elevated in several diseases processes, including insulin resistance, folate deficiency, and hypotension. PBLD has been proved to be involved in gastric carcinogenesis, PBLD expression was attenuated in gastric cancer (GC) tissues, and negatively regulated the growth and invasion in gastric cancer cell-lines by inhibiting TGF-β1-induced EMT (Epithelial-Mesenchymal Transition).(PMID: 11355021, 17929853, 23687415, 26594798)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PBLD antibody CL488-68317 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
