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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CoraLite® Plus 488-conjugated PBLD Monoclonal anti (CL488-68317)

    IF Staining of HEK-293 using CL488-68317

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 PBLD antibody (CL488-68317, Clone: 1G11D4 ) at dilution of 1:200.

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CoraLite® Plus 488-conjugated PBLD Monoclonal antibody

PBLD Monoclonal Antibody for IF/ICC
Cat No. CL488-68317
Clone No.1G11D4

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

IF/ICC

Synonyms

MAWDBP, MAWD-binding protein, MAWD binding protein, MAWBP, EC:5.1.-.-

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68317 targets PBLD in IF/ICC applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PBLD fusion protein Ag23827 相同性解析による交差性が予測される生物種
Full Name phenazine biosynthesis-like protein domain containing
Observed molecular weight30 kDa
GenBank accession numberBC009738
Gene Symbol PBLD
Gene ID (NCBI) 64081
RRIDAB_3672995
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP30039
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PBLD, also termed as MAWBP, was first identified from a human liver cDNA library using a yeast two-hybrid technique by Iriyama et al. As the only representative of phenazine biosynthesis-like protein family, PBLD is widely expressed in human tissues, such as brain, heart, lung, liver, pancreas, kidney, and placenta. Its expression is elevated in several diseases processes, including insulin resistance, folate deficiency, and hypotension. PBLD has been proved to be involved in gastric carcinogenesis, PBLD expression was attenuated in gastric cancer (GC) tissues, and negatively regulated the growth and invasion in gastric cancer cell-lines by inhibiting TGF-β1-induced EMT (Epithelial-Mesenchymal Transition).(PMID: 11355021, 17929853, 23687415, 26594798)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PBLD antibody CL488-68317Download protocol
Standard Protocols
Click here to view our Standard Protocols