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PCCB Monoclonal antibody
PCCB Monoclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
pig, rat, mouse, human
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
1A9A5
Cat no : 66501-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, human heart tissue, HepG2 cells, HSC-T6 cells, pig heart tissue, rat heart tissue, mouse liver tissue |
Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human kidney tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
66501-1-Ig targets PCCB in WB, IHC, IF-P, ELISA applications and shows reactivity with pig, rat, mouse, human samples.
Tested Reactivity | pig, rat, mouse, human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | PCCB fusion protein Ag1608 相同性解析による交差性が予測される生物種 |
Full Name | propionyl Coenzyme A carboxylase, beta polypeptide |
Calculated molecular weight | 58 kDa |
Observed molecular weight | 58 kDa |
GenBank accession number | BC013768 |
Gene symbol | PCCB |
Gene ID (NCBI) | 5096 |
RRID | AB_2881865 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for PCCB antibody 66501-1-Ig | Download protocol |
IHC protocol for PCCB antibody 66501-1-Ig | Download protocol |
IF protocol for PCCB antibody 66501-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |