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  • Featured Product
  • KD/KO Validated

PCCB Monoclonal antibody, PBS Only (Capture/Detector)

PCCB Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A9A5

Cat no : 66501-1-PBS

Synonyms

Propionyl-CoA carboxylase beta chain, mitochondrial, Propanoyl-CoA:carbon dioxide ligase subunit beta, PCCase subunit beta, EC:6.4.1.3, 1A9A5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCCB Monoclonal antibody (66501-1-Ig)

    WB analysis using 66501-1-Ig (same clone as 66501-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66501-1-Ig (PCCB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66501-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PCCB Monoclonal antibody (66501-1-Ig)

    WB analysis using 66501-1-Ig (same clone as 66501-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66501-1-Ig (PCCB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66501-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using PCCB Monoclonal antibody (66501-1-Ig)

    WB analysis of HepG2 using 66501-1-Ig (same clone as 66501-1-PBS)

    WB result of PCCB antibody (66501-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PCCB transfected HepG2 cells. This data was developed using the same antibody clone with 66501-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using PCCB Monoclonal antibody (66501-1-Ig)

    IHC staining of human heart using 66501-1-Ig (same clone as 66501-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66501-1-Ig (PCCB antibody) at dilution of 1:300 (under 10x lens). This data was developed using the same antibody clone with 66501-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using PCCB Monoclonal antibody (66501-1-Ig)

    IHC staining of human heart using 66501-1-Ig (same clone as 66501-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66501-1-Ig (PCCB antibody) at dilution of 1:300 (under 40x lens). This data was developed using the same antibody clone with 66501-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using PCCB Monoclonal antibody (66501-1-Ig)

    IF Staining of human kidney using 66501-1-Ig (same clone as 66501-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using 66501-1-Ig (PCCB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66501-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50335-2

    Cytometric bead array standard curve of MP50335-2

    Cytometric bead array standard curve of MP50335-2, PCCB Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66501-2-PBS. Detection antibody: 66501-1-PBS. Standard:Ag1608. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP50335-3

    Cytometric bead array standard curve of MP50335-3

    Cytometric bead array standard curve of MP50335-3, PCCB Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66501-1-PBS. Detection antibody: 66501-3-PBS. Standard:Ag1608. Range: 0.781-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66501-1-PBS targets PCCB as part of a matched antibody pair:

MP50335-2: 66501-2-PBS capture and 66501-1-PBS detection (validated in Cytometric bead array)

MP50335-3: 66501-1-PBS capture and 66501-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PCCB fusion protein Ag1608 相同性解析による交差性が予測される生物種
Full Name propionyl Coenzyme A carboxylase, beta polypeptide
Calculated molecular weight 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC013768
Gene symbol PCCB
Gene ID (NCBI) 5096
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.