Validation Data Gallery
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Tested Applications
| Positive WB detected in | pig kidney tissue, pig liver tissue, rat liver tissue |
| Positive IHC detected in | mouse kidney tissue, human kidney tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66862-1-Ig targets PCK1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
| Tested Reactivity | Human, Mouse, Rat, Pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | PCK1 fusion protein Ag9720 相同性解析による交差性が予測される生物種 |
| Full Name | phosphoenolpyruvate carboxykinase 1 (soluble) |
| Calculated molecular weight | 622 aa, 69 kDa |
| Observed molecular weight | 65-69 kDa |
| GenBank accession number | BC023978 |
| Gene Symbol | PCK1 |
| Gene ID (NCBI) | 5105 |
| RRID | AB_2882200 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P35558 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PCK1(Phosphoenolpyruvate carboxykinase, cytosolic) is also named as PEPCK1 and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. It is also a main control point for the regulation of gluconeogenesis. In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PCK1 antibody 66862-1-Ig | Download protocol |
| IHC protocol for PCK1 antibody 66862-1-Ig | Download protocol |
| IF protocol for PCK1 antibody 66862-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |