PCK1 Monoclonal antibody
PCK1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat, Pig
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
2F11B5
Cat no : 66862-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | pig kidney tissue, pig liver tissue, rat liver tissue |
Positive IHC detected in | mouse kidney tissue, human kidney tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
66862-1-Ig targets PCK1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
Tested Reactivity | Human, Mouse, Rat, Pig |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | PCK1 fusion protein Ag9720 相同性解析による交差性が予測される生物種 |
Full Name | phosphoenolpyruvate carboxykinase 1 (soluble) |
Calculated molecular weight | 622 aa, 69 kDa |
Observed molecular weight | 65-69 kDa |
GenBank accession number | BC023978 |
Gene symbol | PCK1 |
Gene ID (NCBI) | 5105 |
RRID | AB_2882200 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PCK1(Phosphoenolpyruvate carboxykinase, cytosolic) is also named as PEPCK1 and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. It is also a main control point for the regulation of gluconeogenesis. In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD).
Protocols
Product Specific Protocols | |
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WB protocol for PCK1 antibody 66862-1-Ig | Download protocol |
IHC protocol for PCK1 antibody 66862-1-Ig | Download protocol |
IF protocol for PCK1 antibody 66862-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |