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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®555-conjugated PCK1 Monoclonal antibody (CL555-66862)

    IF Staining of HeLa using CL555-66862

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®555 PCK1 antibody (CL555-66862, Clone: 2F11B5 ) at dilution of 1:200.

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CoraLite®555-conjugated PCK1 Monoclonal antibody

PCK1 Monoclonal Antibody for IF/ICC
Cat No. CL555-66862
Clone No.2F11B5

Host / Isotype

Mouse / IgG1

Reactivity

mouse, rat, pig, human

Applications

IF/ICC

Synonyms

PCK1, PEPCK C, PEPCK1, PEPCKC

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®555
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66862 targets PCK1 in IF/ICC applications and shows reactivity with mouse, rat, pig, human samples.

Tested Reactivity mouse, rat, pig, human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PCK1 fusion protein Ag9720 相同性解析による交差性が予測される生物種
Full Name phosphoenolpyruvate carboxykinase 1 (soluble)
Calculated molecular weight 622 aa, 69 kDa
Observed molecular weight 65-69 kDa
GenBank accession numberBC023978
Gene Symbol PCK1
Gene ID (NCBI) 5105
RRIDAB_2934681
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP35558
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PCK1(Phosphoenolpyruvate carboxykinase, cytosolic) is also named as PEPCK1 and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. It is also a main control point for the regulation of gluconeogenesis. In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD).

Protocols

Product Specific Protocols
IF protocol for CL555 PCK1 antibody CL555-66862Download protocol
Standard Protocols
Click here to view our Standard Protocols