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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse testis tissue using PCOTH Polyclonal antibody (24564-1-AP)

    IHC staining of mouse testis using 24564-1-AP (same clone as 24564-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24564-1-AP (PCOTH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24564-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PCOTH Polyclonal antibody (24564-1-AP)

    IHC staining of mouse testis using 24564-1-AP (same clone as 24564-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24564-1-AP (PCOTH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24564-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PCOTH Polyclonal antibody (24564-1-AP)

    IHC staining of human prostate cancer using 24564-1-AP (same clone as 24564-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 24564-1-AP (PCOTH antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24564-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PCOTH Polyclonal antibody (24564-1-AP)

    IHC staining of human prostate cancer using 24564-1-AP (same clone as 24564-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 24564-1-AP (PCOTH antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24564-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using PCOTH Polyclonal antibody (24564-1-AP)

    IF Staining of PC-3 using 24564-1-AP (same clone as 24564-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using PCOTH antibody (24564-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24564-1-PBS in a different storage buffer formulation.

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PCOTH Polyclonal antibody, PBS Only

PCOTH Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 24564-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24564-1-PBS targets PCOTH in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCOTH fusion protein Ag20092 相同性解析による交差性が予測される生物種
Full Name prostate collagen triple helix
Calculated molecular weight 107 aa, 11 kDa
GenBank accession numberBC142999
Gene Symbol PCOTH
Gene ID (NCBI) 542767
RRIDAB_2918084
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ58A44
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PCOTH may be involved in growth and survival of prostate cancer cells through the TAF-Ibeta pathway.