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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCYT1A Recombinant antibody (83100-1-RR)

    WB analysis using 83100-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83100-1-RR (PCYT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PCYT1A Recombinant antibody (83100-1-RR)

    WB analysis using 83100-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83100-1-RR (PCYT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PCYT1A Recombinant antibody (83100-1-RR)

    IF Staining of MCF-7 using 83100-1-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PCYT1A antibody (83100-1-RR, Clone: 230393B4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of A431 cells using PCYT1A Recombinant antibody (83100-1-RR)

    FC experiment of A431 using 83100-1-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human PCYT1A (83100-1-RR, Clone:230393B4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83100-1-RR

    Affinity and Kinetic Characterization of 83100-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83100-1-RR against Human PCYT1A were performed. The affinity constant is below 1 pM.

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PCYT1A Recombinant antibody

PCYT1A Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83100-1-RR
Clone No.230393B4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

PCYT1, CTPCT, CT A, CCT alpha, CCT A

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, NIH/3T3 cells, mouse testis tissue, mouse brain tissue
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83100-1-RR targets PCYT1A in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PCYT1A fusion protein Ag34268 相同性解析による交差性が予測される生物種
Full Name phosphate cytidylyltransferase 1, choline, alpha
Calculated molecular weight42 kDa
Observed molecular weight38-41 kDa
GenBank accession numberNM_005017
Gene Symbol PCYT1A
Gene ID (NCBI) 5130
RRIDAB_3670813
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49585
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PCYT1A antibody 83100-1-RRDownload protocol
IF protocol for PCYT1A antibody 83100-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols