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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated PCYT1A Recombinant antibody

PCYT1A Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230393B4

Cat no : CL488-83100

Synonyms

PCYT1, CTPCT, CT A, CCT alpha, CCT A

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite® Plus 488-conjugated PCYT1A Recombinant a (CL488-83100)

    IF Staining of MCF-7 using CL488-83100

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 PCYT1A antibody (CL488-83100, Clone: 230393B4 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of A431 cells using CoraLite® Plus 488-conjugated PCYT1A Recombinant a (CL488-83100)

    FC experiment of A431 using CL488-83100

    1x10^6 A431 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Pcyt1a Recombinant Antibody (CL488-83100, Clone:230393B4)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83100 targets PCYT1A in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PCYT1A fusion protein Ag34268 相同性解析による交差性が予測される生物種
Full Name phosphate cytidylyltransferase 1, choline, alpha
Calculated molecular weight42 kDa
Observed molecular weight38-41 kDa
GenBank accession numberNM_005017
Gene symbol PCYT1A
Gene ID (NCBI) 5130
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PCYT1A (phosphate cytidylyltransferase 1A, choline), also known as CTPCT. It is expected to be located in the nucleus and cytoplasm, which is enriched in brain, placenta, liver, fetal and adult lung. This gene belongs to the cytidylyltransferase family and is involved in the regulation of phosphatidylcholine biosynthesis. Mutations in this gene are associated with spondylometaphyseal dysplasia with cone-rod dystrophy. The molecular weight of PCYT1A is 41 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PCYT1A antibody CL488-83100Download protocol
FC protocol for CL Plus 488 PCYT1A antibody CL488-83100Download protocol
Standard Protocols
Click here to view our Standard Protocols