• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human PD-1/CD279 Rabbit Recombinant Antibody, PBS Only

PD-1/CD279 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240724G11

Cat no : 98068-1-PBS

Synonyms

CD279, PD1, PD-1, 240724G11, PD 1

Validation Data Gallery

Filter:
  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human PD-1/CD279 Rabbit Recombinant Antibody (98068-1-RR)

    FC experiment of human PBMCs using 98068-1-RR (same clone as 98068-1-PBS)

    1x10^6 unstimulated or PHA stimulated human PBMCs were stained with 0.25 ug Anti-Human PD-1/CD279 Rabbit Recombinant Antibody (98068-1-RR, Clone:240724G11) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were not fixed. This data was developed using the same antibody clone with 98068-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human PD-1/CD279 Rabbit Recombinant Antibody (98068-1-RR)

    FC experiment of human PBMCs using 98068-1-RR (same clone as 98068-1-PBS)

    1x10^6 PHA stimulated human PBMCs were stained with 0.25 ug Anti-Human PD-1/CD279 Rabbit Recombinant Antibody (98068-1-RR, Clone:240724G11)(left) or 0.25 ug PE Anti-Human PD-1/CD279 (J110) (PE-65119, Clone: J110)(right) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), Cells were then stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were not fixed. This data was developed using the same antibody clone with 98068-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human PD-1/CD279 Rabbit Recombinant Antibody (98068-1-RR)

    FC experiment of human PBMCs using 98068-1-RR (same clone as 98068-1-PBS)

    1x10^6 unstimulated (black) or PHA stimulated human PBMCs (red) were stained with 0.25 ug Anti-Human PD-1/CD279 Rabbit Recombinant Antibody (98068-1-RR, Clone:240724G11) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 PHA treated human PBMCs were stained with Isotype Control(blue). Cells were not fixed. This data was developed using the same antibody clone with 98068-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human PD-1/CD279 Rabbit Recombinant Antibody (98068-1-RR)

    FC experiment of human PBMCs using 98068-1-RR (same clone as 98068-1-PBS)

    1x10^6 unstimulated (black) or PHA treated human PBMCs were stained with 0.25 ug Anti-Human PD-1/CD279 Rabbit Recombinant Antibody (98068-1-RR, Clone:240724G11)(red) and PE-Conjugated Goat Anti-Rabbit IgG (H+L) or 0.25 ug PE Anti-Human PD-1/CD279 (J110) (PE-65119, Clone: J110)(green). 1x10^6 PHA treated human PBMCs were stained with Isotype Control(blue) . Cells were not fixed. This data was developed using the same antibody clone with 98068-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98068-1-RR

    Affinity and Kinetic Characterization of 98068-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98068-1-RR against Human PD-1/CD279 were performed. The affinity constant is 0.966 nM.

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 98068-1-PBS is PD-1/CD279 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name programmed cell death 1
Calculated molecular weight 288 aa, 32 kDa
GenBank accession numberBC074740
Gene symbol PD-1
Gene ID (NCBI) 5133
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). It has been reported that PD-1 is heavily glycosylated and migrates with an apparent molecular mass of 47-55 kDa on SDS-PAGE , which is larger than its predicted mass of 32 kDa (PMID: 8671665; 17640856; 17003438).