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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human PD-1/CD279 Rabbit Recombinant Antibody

PD-1/CD279 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240724G11

Cat no : 98068-1-RR

Synonyms

CD279, PD1, PD-1, 240724G11, PD 1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human PD-1/CD279 Rabbit Recombinant Antibody (98068-1-RR)

    FC experiment of human PBMCs using 98068-1-RR

    1x10^6 unstimulated or PHA stimulated human PBMCs were stained with 0.25 ug Anti-Human PD-1/CD279 Rabbit Recombinant Antibody (98068-1-RR, Clone:240724G11) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human PD-1/CD279 Rabbit Recombinant Antibody (98068-1-RR)

    FC experiment of human PBMCs using 98068-1-RR

    1x10^6 PHA stimulated human PBMCs were stained with 0.25 ug Anti-Human PD-1/CD279 Rabbit Recombinant Antibody (98068-1-RR, Clone:240724G11)(left) or 0.25 ug PE Anti-Human PD-1/CD279 (J110) (PE-65119, Clone: J110)(right) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), Cells were then stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human PD-1/CD279 Rabbit Recombinant Antibody (98068-1-RR)

    FC experiment of human PBMCs using 98068-1-RR

    1x10^6 unstimulated (black) or PHA stimulated human PBMCs (red) were stained with 0.25 ug Anti-Human PD-1/CD279 Rabbit Recombinant Antibody (98068-1-RR, Clone:240724G11) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 PHA treated human PBMCs were stained with Isotype Control(blue). Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human PD-1/CD279 Rabbit Recombinant Antibody (98068-1-RR)

    FC experiment of human PBMCs using 98068-1-RR

    1x10^6 unstimulated (black) or PHA treated human PBMCs were stained with 0.25 ug Anti-Human PD-1/CD279 Rabbit Recombinant Antibody (98068-1-RR, Clone:240724G11)(red) and PE-Conjugated Goat Anti-Rabbit IgG (H+L) or 0.25 ug PE Anti-Human PD-1/CD279 (J110) (PE-65119, Clone: J110)(green). 1x10^6 PHA treated human PBMCs were stained with Isotype Control(blue) . Cells were not fixed.

  • Affinity and Kinetic Characterization of 98068-1-RR

    Affinity and Kinetic Characterization of 98068-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98068-1-RR against Human PD-1/CD279 were performed. The affinity constant is 0.966 nM.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98068-1-RR is PD-1/CD279 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name programmed cell death 1
Calculated molecular weight 288 aa, 32 kDa
GenBank accession numberBC074740
Gene symbol PD-1
Gene ID (NCBI) 5133
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). It has been reported that PD-1 is heavily glycosylated and migrates with an apparent molecular mass of 47-55 kDa on SDS-PAGE , which is larger than its predicted mass of 32 kDa (PMID: 8671665; 17640856; 17003438).

Protocols

Product Specific Protocols
FC protocol for PD-1/CD279 antibody 98068-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols