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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PD-L2/B7-DC Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 15.6-1000 pg/mL

Cat No : MP00778-4

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00778-4, Human PD-L2/B7-DCMonoclonal Matched Antibody Pair - PBS only. 83158-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0507. 83158-1-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

    Sandwich ELISA standard curve of MP00778-4

    Sandwich ELISA standard curve of MP00778-4, Human PD-L2/B7-DCMonoclonal Matched Antibody Pair - PBS only. 83158-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0507. 83158-1-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

  • Serum of fifteen individual healthy human donors were measured. The human PD-L2/B7-DC concentration of detected samples was determined to be 13,665.12 pg/mL with a range of 7,054.02 - 18,074.46 pg/mL.

    Sample test of MP00778-4

    Serum of fifteen individual healthy human donors were measured. The human PD-L2/B7-DC concentration of detected samples was determined to be 13,665.12 pg/mL with a range of 7,054.02 - 18,074.46 pg/mL.

  • Serum of fifteen individual healthy human donors were measured. The human PD-L2/B7-DC concentration of detected samples was determined to be 13,665.12 pg/mL with a range of 7,054.02 - 18,074.46 pg/mL.

    Sample test of MP00778-4

    Serum of fifteen individual healthy human donors were measured. The human PD-L2/B7-DC concentration of detected samples was determined to be 13,665.12 pg/mL with a range of 7,054.02 - 18,074.46 pg/mL.

  • For the PBMC supernatant cultured for 4 days, the mean PD-L2/B7-DC concentration was determined to be 14.8 pg/mL in unstimulated PBMC supernatant, 58.7 pg/mL in PHA stimulated PBMC supernatant.

    Sample test of MP00778-4

    For the PBMC supernatant cultured for 4 days, the mean PD-L2/B7-DC concentration was determined to be 14.8 pg/mL in unstimulated PBMC supernatant, 58.7 pg/mL in PHA stimulated PBMC supernatant.

  • Milk of eight individual healthy human donors was measured. The human PD-L2/B7-DC concentration of detected samples was determined to be 394.5 pg/mL with a range of 206.2 - 1,153.2 pg/mL.

    Sample test of MP00778-4

    Milk of eight individual healthy human donors was measured. The human PD-L2/B7-DC concentration of detected samples was determined to be 394.5 pg/mL with a range of 206.2 - 1,153.2 pg/mL.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00778-4 targets PD-L2/B7-DC in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 15.6-1000 pg/mL (Sandwich ELISA);

Capture antibody: PD-L2/B7-DC Recombinant antibody, PBS Only (Capture) 83158-3-PBS (230514C6). 100 μg. Concentration 1 mgl/ml.

Detection antibody: PD-L2/B7-DC Recombinant antibody, PBS Only (Detector) 83158-1-PBS (230514A2). 100 μg. Concentration 1 mgl/ml.

Alternative PD-L2/B7-DC matched antibody pairs: MP00778-1, MP50162-1

Matched pair protein standard: Eg0507

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83158-3-PBS
Tested Reactivity human
Immunogen Recombinant protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name programmed cell death 1 ligand 2
Synonyms PDCD1LG2, PD-L2, CD273, Butyrophilin B7-DC, B7-DC
GenBank accession numberBC074766
Gene symbol PD-L2
UniprotQ9BQ51
Gene ID (NCBI) 80380
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 83158-1-PBS
Tested Reactivity human
Immunogen Recombinant protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name programmed cell death 1 ligand 2
Synonyms PDCD1LG2, PD-L2, CD273, Butyrophilin B7-DC, B7-DC
GenBank accession numberBC074766
Gene symbol PD-L2
UniprotQ9BQ51
Gene ID (NCBI) 80380
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.